A673

85111504, human muscle, Epithelial-like

• 分類程式碼代碼: 41106514

屬性

• product name: A673, 85111504
• 生物源: human muscle
• 增長模式: Adherent
• 染色體組型: Near Diploid
• 形態學: Epithelial-like
• 產品: Transforming growth factor (Tramnsforming Growth Factor (TGF)) - ß
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Human Ewing sarcoma

細胞系描述

Derived from a 15 year old female patient. The cells form colonies on soft agar and tumours in antithymocyte serum treated mice.

The cell line A673 was originally described in 1973 as derived from a rhabdomyosarcoma, however, more recent analysis of the cells using advanced diagnostic techniques determined the cells to have characteristics of Ewing sarcoma rather than rhabdomyosarcoma. See citations for more information about this tumour status.

應用

Tumourigenicity studies

DNA分析

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 8,13
D16S539: 11
D5S818: 11,12
D7S820: 10,12
THO1: 9.3
TPOX: 8
vWA: 15,18

培養基

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:10, i.e., seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.

其他說明

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