BLO-11

90112702, mouse muscle, Fibroblast-like

• 分類程式碼代碼: 41106514

屬性

• 產品名稱: BLO-11, 90112702
• 生物源: mouse muscle
• 包裝: tube of 5 μg 90112702-DNA-5UG; pkg of vial of cells 90112702-1VL
• 增長模式: Adherent
• 染色體組型: 2n = 40
• 形態學: Fibroblast-like
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Mouse Moblo/y muscle hereditary emphysema

細胞系描述

Established from the abdominal wall muscle of a 30 day old male Blotchy mouse (Mo (blo/y)) with hereditary emphysema. The cells are deficient in lysyl oxidase activity and accumulate high levels of intracellular copper. NOR-10 (Sigma Catalogue number. 90112701) was isolated from the abdominal wall muscle of a normal litter mate of the mouse from which BLO-11 was derived, thus it can serve as a matched control for studies with BLO fibroblasts.

應用

Genetic studies

培養基

DMEM + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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