LA-4

90040512, mouse lung, Epithelial-like

• 分類程式碼代碼: 41106514

屬性

• product name: LA-4, 90040512
• 生物源: mouse lung
• 增長模式: Adherent
• 染色體組型: Unstable, range 38-256 chromosomes
• 形態學: Epithelial-like
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Mouse A/He lung adenoma

細胞系描述

Isolated from a urethan-induced lung adenoma of a 28 week old A/He strain mouse. The cells incorporate high levels of 14C-choline into lecithin. C-type particles have been observed in some cultures.

應用

Tumourigenicity studies

培養基

Ham′s F12 + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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