Sf9 Cell Line from Spodoptera frugiperda pupal ovarian tissue

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 89070101, insect ovary, Spherical

• 同義詞: SF-9 Cells
• 分類程式碼代碼: 41106514

屬性

• 生物源: insect ovary
• 包裝: tube of 5 μg 89070101-DNA-5UG; pkg of vial of cells 89070101-1VL
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Spherical
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Spodoptera frugiperda pupal ovarian tissue

細胞系描述

Derived from pupal ovarian tissue of spodoptera frugiperda. The cells are highly susceptible to Baculovirus infection and are used in the production of protein products genetically manipulated into Baculovirus vector systems.

應用

Sf9 cell line has been used for Baculovirus viral studies. It has also been used for in vitro kynurenine-3-monooxygenase (KMO) inhibition assay.

培養基

TC100 + 2mM Glutamine. (The medium can be supplemented with 10% Foetal Bovine Serum (FBS) if required).

例行更新培養

Maintain cultures between 3x100,000 to 1x1,000,000 cells/ml; 27°C. Gently resuspend cells in old culture medium by dislodging the cells by pipetting, or gentle agitation, and resuspend in fresh medium. Cells should stick to the culture flask. Freeze in 9% DMSO/91% FBS. Cultures may take up to 10 days to recover normal growth characteristics following cryopreservation and resuscitation.

其他說明

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