Sf21

5022801, insect ovary, Spherical

• 分類程式碼代碼: 41106514

屬性

• product name: Sf21, 05022801
• 生物源: insect ovary
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Spherical
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Spodoptera frugiperda pupal ovarian tissue

細胞系描述

Derived from pupal ovarian tissue of spodoptera frugiperda. The cells are highly susceptible to Baculovirus infection and are used in the production of protein products genetically manipulated into Baculovirus vector systems. They were derived at the USDA Insect Pathology Laboratory from a cell line originating from pupal ovarian tissue of the fall army worm, Spodoptera frugiperda. Sf21 cells have greater range in size and have a doubling time of 24 hr compared to 48-72 hr for Sf9 cells.

應用

Protein expression using the Baculovirus vector system

培養基

SF90011 + 10% Foetal Bovine Serum (FBS)

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 5x10,000 cells/cm²; 28 C. Gently resuspend cells in old culture medium by dislodging the cells by pipetting, or gentle agitation, centrifuge suspension and resuspend cell pellet in fresh medium.

其他說明

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