H4II

89042702, rat skin, Epithelial

• 分類程式碼代碼: 41106514

屬性

• product name: H4II, 89042702
• 生物源: rat skin
• 增長模式: Adherent
• 染色體組型: 52 chromosomes
• 形態學: Epithelial
• 產品: Albumin, transferrin, prothrombin
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Rat skin Hepatocellular carcinoma

細胞系描述

From H-35 Reuber hepatoma induced by chemical carcinogenesis in an AxC rat . Tyrosine aminotransferase positive. Synthesises several serum proteins including albumin, transferrin and prothrombin. (Same as H4-11-E-C3 (Sigma Catalogue number. 85061112)).

應用

Gene expression analysis.

培養基

Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

例行更新培養

Split semi-confluent [60%-70%] cultures 1:100 i.e. seeding at 2x100 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Saturation density is reached at 15x1000,000 cells/10cm² dish.

其他說明

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