C32

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 87090201, human skin, Epithelial

• 分類程式碼代碼: 41106514

屬性

• 生物源: human skin
• 包裝: tube of 5 μg 87090201-DNA-5UG; pkg of vial of cells 87090201-1VL
• 增長模式: Adherent
• 染色體組型: Modal no. 45
• 形態學: Epithelial
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Human amelanotic melanoma

細胞系描述

C32 belongs to the C32r type cultures with the marker M2r substituting the original ring marker M2R. Tumours can be produced in nude mice after 1-2 months (1,000,000 cells injected).

應用

Tumourigenicity studies

培養基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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