H4-II-E-C3

85061112, rat liver, Epithelial

• 分類程式碼代碼: 41106514

屬性

• product name: H4-II-E-C3, 85061112
• 生物源: rat liver
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Epithelial
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Rat liver hepatoma

細胞系描述

From H-35 Reuber hepatoma. Tyrosine aminotransferase positive. Synthesises several serum proteins including albumin, transferrin, prothrombin. The presence of type C virus has been shown and can readily be verified by electron microscopy.

應用

Enzymatic and virus studies: Type C virus

培養基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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