Fao Cell Line from rat

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 89042701, rat liver, Hepatocyte

• 分類程式碼代碼: 41106514

屬性

• 生物源: rat liver
• 包裝: tube of 5 μg 89042701-DNA-5UG; pkg of vial of cells 89042701-1VL
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Hepatocyte
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Rat hepatoma

細胞系描述

A differentiated cell line derived from H4-11-E-C3 (Sigma catalogue no. 85061112). A versatile tool for studying regulation of mono-oxygenase activities and analysing their role in the activation of xenobiotics e.g. carcinogens, drugs and pesticides.

應用

Differentiation and enzymatic studies

培養基

Coon′s modified Ham′s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) or kaign′s modified Ham′s F12 + 45mg/l asorbic acid +18mg/1 inositol + 10% FBS +2mM Glutamine

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂ 37°C.

其他說明

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