BC3H1

86093001, mouse brain, Fibroblast

• 分類程式碼代碼: 41106514
• 生物源: mouse brain
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Fibroblast
• 產品: Acetylcholine
• 受體: Not specified
• 相關疾病: cancer

屬性

• 產品名稱: BC3H1, 86093001
• 生物源: mouse brain
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Fibroblast
• 產品: Acetylcholine
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Mouse brain tumour

細胞系描述

Established in 1974 from a mouse brain neoplasm tumour induced by nitrosoethylurea, possess many properties characteristic of smooth muscle. Since then it has been suggested that BC3H1 is a skeletal line of ectodermal origin that is defective for commitment to terminal differentiation. The two distinct morphologies typical of this cell line are large spheroid cells and smooth-muscle-like cells. The fusiform is the more common.

應用

Acetylcholine receptor studies.

培養基

DMEM + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

例行更新培養

Spilt confluent cultures 1:3 to 1:10 i.e. seeding at 1-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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