AtT20

87021902, mouse brain, Small round cells growing as large aggregates

• 分類程式碼代碼: 41106514

屬性

• product name: AtT20, 87021902
• 生物源: mouse brain
• 增長模式: Aggregates in suspension
• 染色體組型: Diploid
• 形態學: Small round cells growing as large aggregates
• 產品: Adrenocorticotropic hormone (ACTH)
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Mouse LAF1 pituitary gland tumour

細胞系描述

Derived after alternate passage of LAF1 mouse pituitary tumour cells as tumours in animals and in cell culture. Cells produce adrenocorticotropic hormone (ACTH) at approximately the rate of 1000mU/mg of cell protein per week.

應用

Hormone studies

培養基

Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS). Cells grow in large aggregates.

例行更新培養

Allow clusters to settle and remove all but 5 to 10 ml of medium. Gently transfer the clusters to new flasks and add fresh medium. A subcultivation ratio of 1:2 is recommended. Cells grow in large viable clusters making counting difficult. A seeding densi

其他說明

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