推薦產品
product name
b.End3, 96091929
生物源
mouse brain
存貨情形
available only in EU
增長模式
Adherent
染色體組型
Not specified
形態學
Endothelial-like
產品
Not specified
受體
Not specified
技術
cell culture | mammalian: suitable
相關疾病
cancer
運輸包裝
dry ice
儲存溫度
−196°C
細胞系來源
Mouse SV129 brain endothelioma
細胞系描述
The cell line b.End3 has been established from brain endothelial cells of SV129 mice. Immortalisation has been carried out by infection of primary cells with retrovirus coding for the Polyoma virus middle T-antigen. b.End3 cells are positive for endothelial specific proteins (PECAM-1, Endoglin, MECA-32, Flk-1) tested by FACS. Inflammatory cytokines induce the expression of proteins such as ICAM-1, VCAM-1 and E-selectin. Injection into mice causes development of haemangiomas.
應用
Study of endothelial cell growth, differentiation and influence of oncogenes.
培養基
DMEM + 2mM Glutamine + 5μM 2-Mercaptoethanol (2ME) + 1mM Sodium Pyruvate (NaP) + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
例行更新培養
Split sub-confluent cultures (70-80%) 1:4 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
其他說明
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分析證明 (COA)
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