WERI

6070602, human eye, Grape like clusters of round cells

• 分類程式碼代碼: 41106514

屬性

• product name: WERI, 06070602
• 生物源: human eye
• 增長模式: Semi-adherent
• 染色體組型: Not specified
• 形態學: Grape like clusters of round cells
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Human Retinoblastoma

細胞系描述

Human retinoblastoma cell line derived from a female Caucasian. The cells are semi-adherent, some neuronal differentiation can occur when subculturing. Tumourigenic in rabbits.

培養基

Iscove′s Modified DMEM + 2mM Glutamine + 15% Foetal Bovine Serum (FBS) + 10ug/ml Insulin + 55uM 2-Mercaptoethanol

例行更新培養

Split subconfluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-3 x100,000 cells/ml; 5% CO₂; 37°C. Saturation density: 2 x 1000,000 cells/ml. Cells are semi-adherent. Scrape cells from surface; do not use trypsin.

其他說明

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