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C9743

Sigma-Aldrich

Anti-CNPase antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

同義詞:

Anti-2′,3′-Cyclic nucleotide 3′-phosphodiesterase, Anti-CNP1

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.41
共軛:
unconjugated
application:
FACS
IHC
WB
無性繁殖:
polyclonal
物種活性:
human, mouse, rat
citations:
10
技術:
flow cytometry: 1:200-1:500
immunohistochemistry: 1:100-1:200
western blot: 1:500-1:1,000

生物源

rabbit

品質等級

共軛

unconjugated

抗體表格

affinity isolated antibody

抗體產品種類

primary antibodies

無性繁殖

polyclonal

形狀

buffered aqueous solution

分子量

antigen 46-48 kDa

物種活性

human, mouse, rat

濃度

~1 mg/mL

技術

flow cytometry: 1:200-1:500
immunohistochemistry: 1:100-1:200
western blot: 1:500-1:1,000

UniProt登錄號

運輸包裝

dry ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

基因資訊

human ... CNP(1267)
mouse ... Cnp(12799)
rat ... Cnp(25275)

免疫原

synthetic peptide corresponding to residues 122-136 of human CNPase. The sequence is 93% identical in mouse and rat.

應用

Anti-CNPase antibody produced in rabbit is suitable for flow cytometry at a working dilution of 1:200-1:500, immunohistochemistry at 1:100-1:200 and western blotting at 1:500-1:1000. It was used at a working dilution of 1:1000 as a primary antibody in western blotting of cerebrospinal fluid samples in a study.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

生化/生理作用

CNPase (2′,3′-cyclic nucleotide 3′-phosphodiesterase) is associated with brain tubulin and acts as a microtubule-associated protein in promoting microtubule assembly and distribution. It is expressed primarily in committed oligodendroglial progenitors and terminally-differentiated oligodendrocytes and may be used as a marker of cell differentiation in the CNS.

外觀

solution in phosphate buffered saline, containing 0.02% sodium azide.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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T Ishii et al.
Neuroscience letters, 268(3), 131-134 (1999-07-16)
We have previously reported that human amniotic epithelial (HAE) cells expressed neuronal and glial cell markers using immunostaining and western blotting. To study the expression system of these cell markers in HAE cells, we investigated the expression of mRNA for
Irum Naureen et al.
Fluids and barriers of the CNS, 10(1), 34-34 (2013-12-20)
In hydrocephalus an imbalance between production and absorption of cerebrospinal fluid (CSF) results in fluid accumulation, compression and stretching of the brain parenchyma. In addition, changes in CSF composition have a profound influence on the development and function of the
2',3'-cyclic nucleotide-3'-phosphohydrolase and signal transduction in central nervous system myelin.
R J Thompson
Biochemical Society transactions, 20(3), 621-626 (1992-08-01)
A J Douglas et al.
Annals of human genetics, 56(Pt 3), 243-254 (1992-07-01)
Human brain cDNA clones for the myelin associated enzyme 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) have been isolated and sequenced. The only 5' untranslated region (UTR) sequence found was that of a human CNPII mRNA, with no direct evidence for a CNPI
S M Staugaitis et al.
Journal of neuroscience research, 25(4), 556-560 (1990-04-01)
The 46 kD isoform of the 2'3'-cyclic nucleotide 3'-phosphodiesterase (CNPI) was expressed in HeLa cells by transfection of its cDNA clone. The distribution of this polypeptide as mapped by indirect immunofluorescence and conventional epifluorescence microscopy appeared diffuse and generally uniform

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