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重要文件

94403

Sigma-Aldrich

Hoechst 33258 溶液

1 mg/mL in H2O, ≥98.0% (HPLC)

同義詞:

双苯并咪唑H 33258溶液, 赫斯特荧光染料 33258 溶液

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About This Item

經驗公式(希爾表示法):
C25H24N6O · 3HCl
CAS號碼:
分子量::
533.88
MDL號碼:
分類程式碼代碼:
12171500
PubChem物質ID:
NACRES:
NA.32

化驗

≥98.0% (HPLC)

形狀

liquid

濃度

1 mg/mL in H2O

溶解度

DMF: soluble
H2O: soluble

螢光

λex 355 nm; λem 465 nm in TE buffer; DNA

儲存溫度

2-8°C

SMILES 字串

Cl.Cl.Cl.CN1CCN(CC1)c2ccc3[nH]c(nc3c2)-c4ccc5nc([nH]c5c4)-c6ccc(O)cc6

InChI

1S/C25H24N6O.3ClH/c1-30-10-12-31(13-11-30)18-5-9-21-23(15-18)29-25(27-21)17-4-8-20-22(14-17)28-24(26-20)16-2-6-19(32)7-3-16;;;/h2-9,14-15,32H,10-13H2,1H3,(H,26,28)(H,27,29);3*1H

InChI 密鑰

SMNPLAKEGAEPJD-UHFFFAOYSA-N

一般說明

赫斯特 33258,也被称为双联苯亚胺,是一种蓝色荧光染料。它被归类为一种染色体染剂,可与DNA双螺旋的小沟结合,识别一组四对A-T碱基对。

應用

赫斯特 33258被用于荧光法估算样品中DNA浓度,这种方法比分光光度法更好,它可以测定纳克级DNA。并且,由于赫斯特 33258适用于识别细胞培养基中的支原体污染,由于其高荧光性。

儲存類別代碼

10 - Combustible liquids

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Measuring the concentration of DNA using fluorometry is more sensitive than spectrophotometry, allowing the detection of nanogram quantities of DNA. In this assay, DNA preparations of known and unknown concentrations are incubated with the fluorochrome Hoechst 33258. Absorption values for
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