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Key Documents

C2888

Sigma-Aldrich

乙酰胆碱酯酶 来源于电鳗

Type V-S, lyophilized powder, ≥1,000 units/mg protein

同義詞:

AChE, 乙酰胆碱乙酰水解酶, 胆碱酯酶,乙酰基

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About This Item

CAS號碼:
酶委員會編號:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.32

生物源

Electrophorus electricus

品質等級

種類

Type V-S

形狀

lyophilized powder

比活性

≥1,000 units/mg protein

分子量

280 kDa

成份

Protein, ≥45% biuret

pI 

~5.3

溶解度

Tris buffer: 1 mg/mL (0.02 M Tris buffer, pH 7.5)

儲存溫度

−20°C

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一般說明

分子量: 280 kDa
等电点: 5.5
消光系数: E1%= 18.0(280 nm)

来自 Electrophorus electricus 的乙酰胆碱酯酶是由 4 个相等的亚基组成的四聚体,每个亚基 70kDa。每个子单元包含一个活性位点。该酶是含有己糖胺的糖蛋白。

應用

电鳗乙酰胆碱酯酶已用于:
  • 乙酰胆碱酯酶活性检测
  • 研究其对爪蟾胚胎海马体细胞的影响
  • 研究乙酰胆碱抑制剂对突触发育和可塑性的影响

生化/生理作用

乙酰胆碱酯酶(AChE)的活性表现于其可降解神经肌肉接头和胆碱能神经元突触间隙处的乙酰胆碱。该酶参与一氧化氮信号通路。
体内乙酰胆碱的主要降解酶。 将乙酰胆碱 + H2O 转化为胆碱+乙酸。

注意

对于稀释溶液(<1 mg/ml),加入 1mg/ml BSA 以稳定。在稀释的中性磷酸盐缓冲液中含有至少 1mg 蛋白质/ml 的溶液在冷藏至少 6 个月后是稳定的。

單位定義

一个单元将在 pH8.0,37℃下,每分钟将 1.0 μmole 乙酰胆碱水解成胆碱和乙酸盐。

外觀

含 Tris 缓冲盐的冻干粉

分析報告

使用乙酰胆碱作为底物获得的活性是用丁基胆碱获得的活性的 30-100 倍,使用来自电鳗的乙酰胆碱酯酶。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析證明 (COA)

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Over the last decades, antifouling coatings containing biocidal compounds as active ingredients were used to prevent biofouling, and eco-friendly alternatives are needed. Previous research from our group showed that polymethoxylated chalcones and glycosylated flavones obtained by synthesis displayed antifouling activity
Silvia Olivera et al.
Molecular and cellular neurosciences, 23(1), 96-106 (2003-06-12)
Here we show that chronic application of low concentrations (0.01-0.05 U/ml) or a single application of 1-5 U/ml acetylcholinesterase (AChE) promotes the extension of neuronal processes, synapse formation, and alpha-amino-3-hydroxy-5-methylisoxazolepropionate receptor (AMPAR) surface expression in both embryonic and postnatal hippocampal
Daniel A S Kitagawa et al.
Journal of enzyme inhibition and medicinal chemistry, 36(1), 1370-1377 (2021-06-22)
Organophosphorus poisoning caused by some pesticides and nerve agents is a life-threating condition that must be swiftly addressed to avoid casualties. Despite the availability of medical countermeasures, the clinically available compounds lack a broad spectrum, are not effective towards all
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ChemMedChem, 12(13), 1055-1069 (2017-06-13)
Four mononuclear [(L-L)2 Ru(tatpp)]2+ and two dinuclear [(L-L)2 Ru(tatpp)Ru(L-L)2 ]4+ ruthenium(II) polypyridyl complexes (RPCs) containing the 9,11,20,22-tetraazatetrapyrido[3,2-a:2',3'-c:3'',2''-l:2''',3'''-n]pentacene (tatpp) ligand were synthesized, in which L-L is a chelating diamine ligand such as 2,2'-bipyridine (bpy), 1,10-phenanthroline (phen), 3,4,7,8-tetramethyl-1,10-phenanthroline (Me4 phen) or 4,7-diphenyl-1,10-phenanthroline
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Biosensors, 11(11) (2021-11-26)
A novel, integrated experimental and modeling framework was applied to an inhibition-based bi-enzyme (IBE) electrochemical biosensor to detect acetylcholinesterase (AChE) inhibitors that may trigger neurological diseases. The biosensor was fabricated by co-immobilizing AChE and tyrosinase (Tyr) on the gold working

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