推薦產品
一般說明
分子量: 280 kDa
等电点: 5.5
消光系数: E1%= 18.0(280 nm)
来自 Electrophorus electricus 的乙酰胆碱酯酶是由 4 个相等的亚基组成的四聚体,每个亚基 70kDa。每个子单元包含一个活性位点。该酶是含有己糖胺的糖蛋白。
等电点: 5.5
消光系数: E1%= 18.0(280 nm)
来自 Electrophorus electricus 的乙酰胆碱酯酶是由 4 个相等的亚基组成的四聚体,每个亚基 70kDa。每个子单元包含一个活性位点。该酶是含有己糖胺的糖蛋白。
應用
电鳗乙酰胆碱酯酶已用于:
- 乙酰胆碱酯酶活性检测
- 研究其对爪蟾胚胎海马体细胞的影响
- 研究乙酰胆碱抑制剂对突触发育和可塑性的影响
生化/生理作用
乙酰胆碱酯酶(AChE)的活性表现于其可降解神经肌肉接头和胆碱能神经元突触间隙处的乙酰胆碱。该酶参与一氧化氮信号通路。
体内乙酰胆碱的主要降解酶。 将乙酰胆碱 + H2O 转化为胆碱+乙酸。
注意
对于稀释溶液(<1 mg/ml),加入 1mg/ml BSA 以稳定。在稀释的中性磷酸盐缓冲液中含有至少 1mg 蛋白质/ml 的溶液在冷藏至少 6 个月后是稳定的。
單位定義
一个单元将在 pH8.0,37℃下,每分钟将 1.0 μmole 乙酰胆碱水解成胆碱和乙酸盐。
外觀
含 Tris 缓冲盐的冻干粉
分析報告
使用乙酰胆碱作为底物获得的活性是用丁基胆碱获得的活性的 30-100 倍,使用来自电鳗的乙酰胆碱酯酶。
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。
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