跳轉至內容
Merck
全部照片(3)

Key Documents

A6941

Sigma-Aldrich

醇氧化酶 来源于博伊丁假丝酵母

lyophilized powder, 5-15 units/mg protein

同義詞:

酒精:氧氧化还原酶

登入查看組織和合約定價


About This Item

CAS號碼:
酶委員會編號:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

生物源

fungus (Candida boidinii)

品質等級

形狀

lyophilized powder

比活性

5-15 units/mg protein

分子量

octomer 600 kDa by sedimentation equilibrium

溶解度

100 mM potassium phosphate, pH 7.5: soluble 1.0 mg/mL at 25 °C (Cold)

儲存溫度

−20°C

尋找類似的產品? 前往 產品比較指南

應用

醇氧化酶用于将短链、伯、脂肪醇催化成各自的醛 。可用于研究甲醇代谢的酵母菌,如 念珠菌 毕赤酵母 汉逊酵母 。研究蛋白质向过氧化物酶体的转位非常有用。产品 A6941 来自 布氏念珠菌 ,以冻干粉形式提供。

生化/生理作用

醇氧化酶对甲醇具有最高的亲和力。 亲和力随烷基(R)基团链长的增加而降低。酒精氧化酶主要定位在过氧化物酶体中,但也存在于细胞质中。 酒精氧化酶是一个600 kDa的同型黄素黄素蛋白,具有8个相等的74 kDa亚基,每个都含有黄素腺嘌呤二核苷酸 (FAD)分子。该酶对仲醇,叔醇或芳族醇几乎没有活性。 或链长超过5个碳的脂肪族醇。该产品活性的pH范围为6.5-8.5,最佳pH为7.5。

單位定義

一个单位将在 pH 7.5 下在 25°C 下每分钟将 1.0μmole 甲醇氧化成甲醛。

外觀

含有磷酸钾缓冲盐、DTE 和稳定剂

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。

已經擁有該產品?

您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

Alcohol oxidase from Candida boidinii.
H Sahm et al.
Methods in enzymology, 89 Pt D, 424-428 (1982-01-01)
B Vinet
Clinical chemistry, 33(12), 2204-2208 (1987-12-01)
This method for the specific determination of methanol in serum is based on the following two reactions: (formula; see text) Alcohol oxidase is not specific: it converts all lower alcohols to their corresponding aldehydes; however, formaldehyde dehydrogenase is specific and
Yu Sasano et al.
FEMS yeast research, 10(5), 535-544 (2010-05-25)
We identified a gene, designated TRM2, responsible for methanol-inducible gene expression in the methylotrophic yeast Candida boidinii. The encoded protein Trm2p contains two C(2)H(2)-type zinc finger motifs near the N terminus and shows high similarity to Saccharomyces cerevisiae Adr1p and
Ping Zhang et al.
Applied and environmental microbiology, 76(18), 6108-6118 (2010-07-27)
In this work, the identification and characterization of two hexose transporter homologs in the methylotrophic yeast Pichia pastoris, P. pastoris Hxt1 (PpHxt1) and PpHxt2, are described. When expressed in a Saccharomyces cerevisiae hxt-null mutant strain that is unable to take
Yasutaka Sasaki et al.
Journal of bioscience and bioengineering, 110(2), 147-151 (2010-06-16)
We have cloned a gene encoding an alcohol oxidase (AOD) specific to aldehyde alcohols from Paenibacillus sp. AIU 311. The AOD gene contains an open reading frame consisting of 618 nucleotides corresponding to 205 amino acid residues. The deduced amino

條款

This procedure may be used for all Alcohol Oxidase products.

我們的科學家團隊在所有研究領域都有豐富的經驗,包括生命科學、材料科學、化學合成、色譜、分析等.

聯絡技術服務