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Merck
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重要文件

A6017

Sigma-Aldrich

ω-Aminohexyl–Agarose

saline suspension

同義詞:

Omega-Aminohexyl-Agarose, 1,6-Diaminohexane–Agarose (N-linked), 6-Aminohexyl–Agarose

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About This Item

CAS號碼:
MDL號碼:
分類程式碼代碼:
23151817
NACRES:
NA.56

生物源

plant

品質等級

形狀

saline suspension

標籤範圍

≥5 μmol per mL

技術

affinity chromatography: suitable

基質

cross-linked 4% beaded agarose

基質活化

cyanogen bromide

基質結合

amino of 1,6-diaminohexane

基質墊片

1 atom

容量

≥5 mg/mL binding capacity (bovine serum albumin)

適合性

suitable for chromatography

儲存溫度

2-8°C

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相關類別

一般說明

Hydrophobic ligands can serve as potentselective adsorbents. Both ω-aminoalkyl and alkyl agaroses can interact with the hydrophobic regions found in various proteins. The ω-aminoalkyl agarose product offered here consists of activated agarose with 1,6-diaminohexane covalently attached to one of its amine groups.

應用

ω-Aminohexyl–Agarose has been used in the conjugation of fatty acids with chain length from two carbons to 20 for fatty acyl beads pull-down experiments.
ω-aminohexyl–agarose has been used for coupling of serotype b-specific carbohydrate antigen (SbAg) of Actinobacillus actinomycetemcomitans Y4 for isolation of anti-SbAg antibodies using protein chromatography.

生化/生理作用

ω-aminoalkyl-agaroses are used in chromatography. In these, the protein is retained by lipophilic association between the hydrocarbon side chains on the agarose and hydrophobic pockets in the protein.

外觀

Suspension in 0.5 M NaCl containing preservative

其他說明

For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.

象形圖

Corrosion

訊號詞

Danger

危險聲明

危險分類

Eye Dam. 1 - Skin Irrit. 2

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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N Margolin et al.
Preparative biochemistry, 21(2-3), 163-173 (1991-01-01)
A method is described to purify recombinant HIV-1 protease from soluble extracts of Escherichia coli. The isolation involves QAE-Sepharose anion exchange chromatography, hexyl agarose hydrophobic interaction chromatography, MonoS cation exchange chromatography, and Superose 6 size exclusion chromatography. Approximately 100 micrograms
Julio Blanco et al.
Acta crystallographica. Section D, Biological crystallography, 58(Pt 2), 352-354 (2002-01-25)
Aspartokinase III catalyzes the commitment step in the aspartate metabolism pathway, the phosphorylation of aspartic acid. The Escherichia coli enzyme has been crystallized in the presence of its natural substrate (aspartic acid) and Mg-ADP and diffraction data has been collected
Isa S Duarte et al.
Artificial organs, 30(8), 606-614 (2006-08-17)
This work investigated the potential use of an alternative adsorbent to anti-immunoglobulin E (IgE)-agarose for IgE selective adsorption therapy. A screening of several commercially available adsorbents (Concanavalin A, Lens culinaris[Lc], d-tryptophan, poly-l-lysine, and aminohexyl immobilized on agarose) was done through
Rapid isolation of microbial milk-clotting enzymes by N-acetyl (or N-isobutyryl)-pepstatin--Aminohexylagarose.
H Kobayashi et al.
Analytical biochemistry, 122(2), 308-312 (1982-05-15)
A H Bakker et al.
Protein engineering, 8(3), 293-300 (1995-03-01)
Tissue-type plasminogen activator (t-PA) is composed of structurally autonomous domains. From the N-terminus of t-PA, a finger-like domain (F), an epidermal growth factor-like domain (G), two kringle domains (K1 and K2) and a serine protease domain (P) can be discerned.

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