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42395

Sigma-Aldrich

丹宁酶 来源于无花果曲霉

powder, white, ≥150 U/g

同義詞:

单宁酰基水解酶

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About This Item

CAS號碼:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

生物源

Aspergillus sp. (A. ficuum)

品質等級

形狀

powder

比活性

≥150 U/g

雜質

25 mM potassium phosphate
250 mM NaCl
50% glycerol

顏色

white

儲存溫度

2-8°C

一般說明

丹宁酶是一种由植物、细菌、真菌和酵母等多种生物产生的酶。这种酶也存在于植物来源中,例如富含丹宁的蔬菜,尤其是在叶子、果实、树枝和树皮中。

應用

来自 黑曲霉 的丹宁酶被用于:
  • 作为标准品测定细菌中分离的丹宁酶活性
  • 研究其对细胞膜合成的单宁酸的抑制作用
  • 通过酶水解葡萄皮和葡萄籽获得原花青素(PA)

生化/生理作用

丹宁酶催化位于复合单宁、没食子单宁和没食子酸酯中的酯键,导致没食子酸的释放。这种酶可用作啤酒、茶、葡萄酒和果汁制造中的澄清剂,以及处理单宁污染的农业废物和工业废水。

單位定義

1 U 相当于在 pH 4.7 和 30°C 下 310 nm 处的吸光度每分钟变化 1.0 的酶量(以单宁酸为底物,最终体积 3 mL)

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


分析證明 (COA)

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Arijit Jana et al.
Applied biochemistry and biotechnology, 167(5), 1254-1269 (2012-01-25)
Tannase production by newly isolated Penicillium purpurogenum PAF6 was investigated by 'one variable at a time' (OVAT) approach followed by response surface methodology (RSM). Tannin-rich plant residues were used as supporting solid substrate and sole carbon source and, among them
Jose Valdo Madeira et al.
Bioprocess and biosystems engineering, 35(3), 477-482 (2011-09-13)
The production of enzymes such as tannases and phytases by solid-state fermentation and their use in animal feed have become a subject of great interest. In the present work, Paecilomyces variotii was used to produce tannase and phytase simultaneously. Solid-state
Jose Valdo Madeira et al.
Bioresource technology, 102(15), 7343-7348 (2011-05-27)
In this work, we introduce a biological detoxification method that converts toxic waste from castor beans into animal feed material. This method simultaneously induces the production of tannase and phytase by Paecilomyces variotii; both enzymes have high levels of activity
Bhakti Bajpai et al.
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 39(4), 708-711 (2008-10-01)
In a new approach to microbial gallic acid production by Aspergillus fischeri MTCC 150, 40gL(-1) of tannic acid was added in two installments during the bioconversion phase of the process (25gL(-1) and 15gL(-1) at 32 and 44h respectively). The optimum
Tadashi Takahashi et al.
Eukaryotic cell, 11(4), 507-517 (2012-01-31)
Loop-out-type recombination is a type of intrachromosomal recombination followed by the excision of a chromosomal region. The detailed mechanism underlying this recombination and the genes involved in loop-out recombination remain unknown. In the present study, we investigated the functions of

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