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重要文件

A9056

Sigma-Aldrich

牛血清白蛋白 来源于牛血清

heat shock fraction, microbiologically tested, ≥96% (agarose gel electrophoresis)

同義詞:

BSA, 牛白蛋白, 牛血清白蛋白

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About This Item

CAS號碼:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352202
NACRES:
NA.27

生物源

bovine

品質等級

化驗

≥96% (agarose gel electrophoresis)

形狀

lyophilized powder

分子量

~66 kDa

純化經由

heat shock fractionation

包裝

poly bottle of

來源

USA origin

技術

cell culture | mammalian: suitable

雜質

≤5.0% Loss on drying

損耗

≤5%

pH值

7

溶解度

water: soluble (40 mg/ml)

UniProt登錄號

異物活動

BT virus, none detected
Microbiological test (pass)
VSV virus, none detected
growth of Borrelia burgdorferi in BSK II medium (Suitable for)

儲存溫度

2-8°C

基因資訊

bovine ... ALB(280717)

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一般說明

Albumin is the most abundant plasma protein in humans. High levels of albumin are associated with dehydration. Albumin turnover is seen in infants with iron deficiency anemia. Serum albumin is a reliable prognostic indicator for liver disease. Oxidative damage of albumin is associated with advanced liver disease.

應用

Bovine Serum Albumin has been used as a supplement in Dulbecco′s modified eagle medium (DMEM). It has also been used in immunostaining of cell arrays.

生化/生理作用

BSA的某些构象和初级序列表位是人类对牛肉和牛奶过敏中的可疑过敏原。

適合性

Suitable for growth of Borrelia burgdorferi in BSK II medium

準備報告

血清白蛋白可能也会被称为Fraction V。该命名惯例取自使用冷乙醇沉淀对血清蛋白进行分级的原始Cohn法。 血清白蛋白可在采用Cohn′法的第五乙醇分级中发现。 从此,有些人便开始使用“Fraction V”这一术语来描述血清白蛋白,无论使用的是什么制备方法。 也有人在使用该术语来描述通过乙醇分级方法而纯化得到血清白蛋白,而这种方法是相对于原始的Cohn法一直在得到高度的改良。Sigma-Aldrich生产和分销从各种主要方法纯化得到的血清白蛋白,包括真正的Cohn分级法、改良的乙醇分级方法、热休克和色谱法。 更多的纯化步骤包括结晶或木炭过滤。
采用热休克分级分离法制备

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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分析證明 (COA)

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Holger Erfle et al.
Nature protocols, 2(2), 392-399 (2007-04-05)
Here, we describe a robust protocol for the reverse transfection of cells on small interfering (siRNA) arrays, which, in combination with multi-channel immunofluorescence or time-lapse microscopy, is suitable for genome-wide RNA interference (RNAi) screens in intact human cells. The automatic
Oxidative damage of albumin in advanced liver disease
Oettl K, et al.
Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1782(7-8), 469-473 (2008)
The role of fresh cow's milk in iron deficiency: I. Albumin turnover in infants with iron deficiency anemia
Woodruff C W and Clark J L
American Journal of Diseases of Children, 124(1), 18-23 (1972)
K K Piotrowska-Tomala et al.
Domestic animal endocrinology, 52, 90-99 (2015-05-04)
The aim of the study was to determine the effects of lipopolysaccharide (LPS), tumor necrosis factor alpha (TNF), interleukin-1-alpha (IL-1α), and nitric oxide donor (NONOate) on both in vivo and in vitro secretion of prostaglandin (PG)E2, PGF2α, leukotriene (LT)B4, and
António Galvão et al.
Mediators of inflammation, 2014, 682193-682193 (2014-08-16)
Metabolic hormones have been associated with reproductive function modulation. Thus, the aim of this study was: (i) to characterize the immunolocalization, mRNA and protein levels of leptin (LEP), Ghrelin (GHR) and respective receptors LEPR and Ghr-R1A, throughout luteal phase; and

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