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重要文件

11207750910

Roche

抗地高辛-罗丹明,Fab片段

from sheep

同義詞:

地高辛配基, 抗-地高辛配基抗体

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About This Item

分類程式碼代碼:
12352203

生物源

sheep

品質等級

共軛

rhodamine conjugate

抗體表格

purified antibody

抗體產品種類

primary antibodies

無性繁殖

polyclonal

形狀

lyophilized (clear, red solution after reconstitution)

包裝

pkg of 200 μg

製造商/商標名

Roche

同型

IgG

儲存溫度

2-8°C

一般說明

地高辛是一种半抗原,可用于核酸标记和检测系统。本产品包含多克隆抗地高辛抗体的Fab片段,与罗丹明偶联。

特異性

绵羊的多克隆抗体显示出与地高辛和地高辛100%的反应性,但与其他类固醇如人雌激素(如雌二醇)或雄激素(如睾酮)没有交叉反应性。

應用

使用抗地高辛-罗丹明Fab片段检测地高辛标记的化合物:
  • 在糖缀合物研究中使用地高辛标记糖
  • 原位杂交中使用荧光
  • 免疫组织细胞化学
  • 原位杂交
  • 脱氧核糖核酸纤维分析

準備報告

地高辛免疫后,用离子交换色谱法纯化绵羊IgG,用免疫吸附法分离特异性IgG。木瓜蛋白酶消化获得的Fab片段通过凝胶过滤纯化,与特定标记缀合,并稳定在缓冲液中。

工作浓度:共轭物的工作浓度取决于应用和底物。以下浓度应作为指导:糖缀合物中地高辛标记糖的检测:20至50 & # 956;g/ml
  • 荧光原位杂交:1-20 & # 956;g/ml
  • 免疫组织细胞化学:20-50 & # 956;g/ml
  • 原位杂交:20-50 & # 956;g/ml

工作溶液:PBS,0.5%牛血清白蛋白,pH 7.4。
1%封闭剂(w/v)、1-5%热灭活胎牛血清(v/v)或绵羊正常血清可用于降低非特异性结合。此外,可将酸碱度提高至8.5至9.0。

储存条件(工作方案):必须制备新鲜物料!

重構

加入1ml双蒸水至终浓度为200μg/ml。

其他說明

仅用于生命科学研究。不可用于诊断。

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象形圖

Exclamation mark

訊號詞

Warning

危險聲明

危險分類

Aquatic Chronic 3 - Skin Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 2

閃點(°F)

does not flash

閃點(°C)

does not flash


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分析證明 (COA)

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