10378445001

Chromozym PK

90% (enzymatic), powder, pkg of 20 mg

• 同義詞: C33H39N8O6COOCH3
• 分類程式碼代碼: 12352204

屬性

• 描述: Molecular Formula: C₃₃H₃₉N₈O₆COOCH₃
• 品質等級: 100
• 化驗: 90% (enzymatic)
• 形狀: powder
• 分子量: M_r 702.9
• 包裝: pkg of 20 mg
• 製造商／商標名: Roche
• 儲存溫度: room temp

描述

一般說明

Chromozym^® PK is a non-radioactive amidolytic chromogenic substrate mostly used in kinetic analysis of plasma kallikrein.

應用

Use Chromozym^® PK as a substrate for the determination of serine proteases, specifically plasma kallikrein in citrated plasma.

品質

Contaminant: <0.5% free 4-nitraniline

序列

Benzoyl-prolyl-phenalanyl-arginine-4-nitranilide-acetate

原則

Chromozym PK is cleaved by plasma kallikrein into a residual peptide and free 4-nitraniline, which is measured at 405 nm. The absorbance difference per minute is used for the determination of the kallikrein activity in U/ml.

準備報告

Activator: Mg²⁺ (K_m = 3.0 mM, pH 7.4, 25 °C); other divalent metal ions (Mn²⁺, Fe²⁺, Zn²⁺)
Working concentration: Approximately 0.5 mM
Storage conditions (working solution): 2 to 8 °C
An aqueous solution of Chromozym PK (1.3 mM) is stable for at least 4 weeks if stored at 2 to 8 °C.

其他說明

仅用于生命科学研究。不可用于诊断。

法律資訊

Chromozym is a registered trademark of Pentapharm AG, Basel

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable