推薦產品
品質等級
形狀
liquid
製造商／商標名
Chemicon®
技術
cell culture | stem cell: suitable
輸入
sample type mesenchymal stem cell(s)
運輸包裝
dry ice
應用
1. Thaw cell culture freezing medium completely and mix well by gently swirling bottle. Keep freezing medium on ice during use.
2. Cells to be frozen should be in late log phase growth.
3. Monolayers will need to be dissociated. After dissociation, cells are resuspended in Mesenchymal Stem Cell Expansion Medium (Cat. No. SCM015) and counted to determine viability and number.
4. Centrifuge cells at 300 x g for 3 min. Remove the medium above the pellet.
5. Resuspend the cells in Mesenchymall Stem Cell Freezing Medium at a concentration of ~4 x 106 cells/mL. Freeze 1 mL of cells/vial. After the cells have been resuspended and aliquoted into appropriate cryogenic storage vials, they can be placed in a freezing container and the normal freeze down procedure should be started within five minutes.
6. Cells must be stored at or below -80°C. For long term storage the cells should be stored in ultra-low temperature freezer (-150°C), or in liquid nitrogen (-196°C).
7. Thawing of cryopreserved cells should be as follows:
a. Do not thaw the cells until the recommended medium and appropriate plasticware and/or glassware are on hand.
b. Thaw cells quickly in a 37°C water bath. Important: Do not vortex the cells.
c. Sterilize vial by rinsing with 70% ethanol.
d. In a laminar flow hood, use a 1 or 2 mL pipette to transfer the cells to a sterile 15 mL conical tube. Be careful to not introduce any bubbles during the transfer process.
e. Using a 10 mL pipette, slowly add dropwise 9 mL of Mesenchymal Stem Cell Expansion Medium (pre-warmed at 37°C) to the 15 mL conical tube. IMPORTANT: Do not add the whole volume of medium at once to the cells. This may result in decreased cell viability due to osmotic shock.
f. Gently mix the cell suspension by slow pipeting up and down twice. Be careful to not introduce any bubbles. IMPORTANT: Do not vortex the cells.
g. Centrifuge the tube at 300 x g for 2-3 minutes to pellet the cells.
h. Decant as much of the supernatant as possible
i. Resuspend the cells in a total volume of 10 mL of Mesenchymal Stem Cell Expansion Medium (pre-warmed at 37°C).
j. Plate the cell mixture onto a 10-cm tissue culture plate.
k. Incubate the cells at 37°C in a 5% CO2 humidified incubator.
l. The next day, exchange the medium with fresh Mesenchymal Stem Cell Expansion Medium (pre-warmed to 37°C). Exchange with fresh medium every two to three days thereafter.
m. When the cells are approximately 80% confluent, they can be dissociated with Accutase® (Cat. No. SCR005) and passaged or alternatively frozen for later use.
2. Cells to be frozen should be in late log phase growth.
3. Monolayers will need to be dissociated. After dissociation, cells are resuspended in Mesenchymal Stem Cell Expansion Medium (Cat. No. SCM015) and counted to determine viability and number.
4. Centrifuge cells at 300 x g for 3 min. Remove the medium above the pellet.
5. Resuspend the cells in Mesenchymall Stem Cell Freezing Medium at a concentration of ~4 x 106 cells/mL. Freeze 1 mL of cells/vial. After the cells have been resuspended and aliquoted into appropriate cryogenic storage vials, they can be placed in a freezing container and the normal freeze down procedure should be started within five minutes.
6. Cells must be stored at or below -80°C. For long term storage the cells should be stored in ultra-low temperature freezer (-150°C), or in liquid nitrogen (-196°C).
7. Thawing of cryopreserved cells should be as follows:
a. Do not thaw the cells until the recommended medium and appropriate plasticware and/or glassware are on hand.
b. Thaw cells quickly in a 37°C water bath. Important: Do not vortex the cells.
c. Sterilize vial by rinsing with 70% ethanol.
d. In a laminar flow hood, use a 1 or 2 mL pipette to transfer the cells to a sterile 15 mL conical tube. Be careful to not introduce any bubbles during the transfer process.
e. Using a 10 mL pipette, slowly add dropwise 9 mL of Mesenchymal Stem Cell Expansion Medium (pre-warmed at 37°C) to the 15 mL conical tube. IMPORTANT: Do not add the whole volume of medium at once to the cells. This may result in decreased cell viability due to osmotic shock.
f. Gently mix the cell suspension by slow pipeting up and down twice. Be careful to not introduce any bubbles. IMPORTANT: Do not vortex the cells.
g. Centrifuge the tube at 300 x g for 2-3 minutes to pellet the cells.
h. Decant as much of the supernatant as possible
i. Resuspend the cells in a total volume of 10 mL of Mesenchymal Stem Cell Expansion Medium (pre-warmed at 37°C).
j. Plate the cell mixture onto a 10-cm tissue culture plate.
k. Incubate the cells at 37°C in a 5% CO2 humidified incubator.
l. The next day, exchange the medium with fresh Mesenchymal Stem Cell Expansion Medium (pre-warmed to 37°C). Exchange with fresh medium every two to three days thereafter.
m. When the cells are approximately 80% confluent, they can be dissociated with Accutase® (Cat. No. SCR005) and passaged or alternatively frozen for later use.
外觀
Serum-containing formulation. Contains 10% DMSO.
儲存和穩定性
Store at -20°C.
法律資訊
Accutase is a registered trademark of Innovative Cell Technologies, Inc.
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
文章
Development of a novel serum-free and xeno-free human mesenchymal stem cell (MSC) osteocyte differentiation media.
新型无血清、无异种人间充质干细胞（MSC）骨细胞分化培养基的开发。
PLTMax Human Platelet Lysate (hPL) is a superior serum-free and xeno-free media supplement alternative to fetal bovine serum (FBS) for human mesenchymal stem cell (MSC) cultures.
我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.
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