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C9249

Sigma-Aldrich

CryoSOfree ™ 无 DMSO 的冷冻保存培养基

greener alternative

同義詞:

cell freezing medium

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.75

形狀

liquid

品質等級

200

環保替代產品特色

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

技術

cell culture | mammalian: suitable
cryopreservation: suitable

環保替代類別

, Aligned

運輸包裝

wet ice

儲存溫度

2-8°C

相關類別

一般說明

悬浮液中的培养细胞可低温储存，最大限度避免冷冻和解冻步骤。CryoSOfree™ 无 DMSO 低温储存培养基储存细胞可使其保持较高活力和生理活性。 建议使用这种培养基储存干细胞、肝细胞、组织样本以及多种类型细胞和成熟细胞系。CryoSOfree™ 无 DMSO 超低温冷冻保存培养基为专利配方，不含 DMSO 或动物蛋白。 配方成分完全，可以立即使用，无需添加剂。
我们致力于为您提供更环保的替代产品，以符合“绿色化学的12项原则”的一项或多项原则要求。相比使用DMSO用于细胞培养物冷冻保存的标准用法，本产品符合本安型化学原则。更多信息，请参阅 此处。

應用

CryoSOfree 是一种化学成分明确的不含 DMSO 的冷冻保存培养基。它不含动物成分，已被证明 能保留干细胞的分化潜能。

特點和優勢

  • 保留干细胞的多能性
  • 低细胞毒性
  • 化学成分明确、无血清、无蛋白
  • 即用型


用 CryoSOfree 冷冻保存后，以下细胞系已恢复 ≥90%：
L929、KB、MC3T3、B16F1、Colon26、CaCO2、HT1080、MG63

準備報告

1 mL CyroSOfree 足以制备 5x105- 5x106 个细胞。

法律資訊

CryoSOfree is a trademark of Sigma-Aldrich Co. LLC

象形圖

Corrosion
GHS05

訊號詞

Warning

危險聲明

H290,H315,H319

危險分類

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

儲存類別代碼

8A - Combustible corrosive hazardous materials

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。

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您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

Kazuaki Matsumura et al.
Biomaterials, 30(27), 4842-4849 (2009-06-12)
Dimethyl sulfoxide (DMSO) has been used for several decades as the most efficient cryoprotective agent (CPA) for many types of cells and tissues in spite of its cytotoxicity and its effects on differentiation. Here we report that polyampholytes with an
Kazuaki Matsumura et al.
Cell transplantation, 19(6), 691-699 (2010-06-08)
Cryoprotective agents (CPAs) such as dimethyl sulfoxide (DMSO), glycerol, ethylene glycol, and propylene glycol have been used for the cryopreservation of cells and tissues. DMSO is the most effective CPA but shows high cytotoxicity and can effect differentiation. ɛ-poly-L-lysine (PLL)
Matthew A Schaller et al.
JCI insight, 6(18) (2021-08-07)
Cell lines are the mainstay in understanding the biology of COVID-19 infection but do not recapitulate many of the complexities of human infection. The use of human lung tissue is one solution for the study of such novel respiratory pathogens.

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Introducing a new cell therapy demands optimal performance in upstream and downstream processing, process development, testing, and manufacturing. World-class products and partners are critical to success, speeding advancement of therapeutics that are effective, safe, and affordable.

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