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Key Documents

PC177L

Sigma-Aldrich

Anti-Serotonin (5-HT) Transporter (602-622) Rabbit pAb

lyophilized, Calbiochem®

同義詞:

Rabbit Anti-Serotonin Transporter, Serotonin Transporter Antibody

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.43

生物源

rabbit

品質等級

抗體表格

serum

抗體產品種類

primary antibodies

無性繁殖

polyclonal

形狀

lyophilized

包含

≤0.1% sodium azide as preservative

物種活性

mouse, rat

製造商/商標名

Calbiochem®

儲存條件

OK to freeze
avoid repeated freeze/thaw cycles

同型

IgG

運輸包裝

ambient

儲存溫度

−20°C

目標翻譯後修改

unmodified

基因資訊

mouse ... Slc6A4(15567)

一般說明

Rabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the serotonin (5-HT) transporter protein.
Recognizes the serotonin (5-HT) transporter in hypothalamus raphe nuclei and spinal cord.
This Anti-Serotonin (5-HT) Transporter (602-622) Rabbit pAb is validated for use in Frozen Sections, Immunofluorescence for the detection of Serotonin (5-HT) Transporter (602-622).

免疫原

Rat
a synthetic peptide corresponding to amino acids 602-622 of rat 5-HT transporter

應用


Frozen Sections (1:10,000-1:15,000; see application references)
Immunoblotting (see comments)
Immunofluorescence (1:200-1:400)

警告

Toxicity: Standard Handling (A)

外觀

Undiluted serum.

重構

Reconstitute the lyophilized antibody with 100 µl of sterile distilled H₂O. Reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Further dilute with sterile PBS or Tris buffer to dilutions no higher than 1:10.

分析報告

Positive Control
Rat naphe nuclei, hypothalamus, cortex, or spinal cord

其他說明

Antibody specificity was examined in the rat cortex, hypothalamus, raphe nuclei, and spinal cord. Staining is completely eliminated by pretreatment of antibody with Serotonin (5-HT) Transporter Control Peptide (Cat. No. PP87) at a concentration of 5 µg/ml. Low levels of glutaraldehyde (0.1%-0.3%) may be used in conjunction with paraformaldehyde for tissue perfusion fixation. The specificity of this antibody was also determined by immunoblotting analysis using rat brain extracts of the cortex, hypothalamus, midbrain, and hindbrain, which specifically detected a single band. Antibody should be titrated for optimal results in individual systems.
Zhou, F.C., et al. 1996. Mol. Brain Res.43, 267.
Blakely, R.D., et al. 1994. J. Exp. Biol.196, 263.
Coccaro, E.F. and Murphy, D.L. (Eds.) 1990. in Serotonin in Major Disorders. The Progress in Psychiatry Series, American Psychiatric Press, Washington, D.C.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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S Nullmeier et al.
The European journal of neuroscience, 33(4), 689-698 (2011-01-14)
Heterozygous reeler mice (HRM), haploinsufficient for reelin, have been proposed to be a genetic mouse model of schizophrenia. Beside behavioural similarities, HRM also demonstrate several neuroanatomical traits similar to patients suffering from schizophrenia. In the present study using immunocytochemical procedures
Diana K Sarko et al.
The Journal of comparative neurology, 519(1), 64-74 (2010-12-02)
Northern grasshopper mice (Onychomys leucogaster) are among the most highly carnivorous rodents in North America. Because predatory mammals may have specialization of senses used to detect prey, we investigated the organization of sensory areas within grasshopper mouse neocortex and quantified
Andrea Forero et al.
Frontiers in cellular neuroscience, 11, 307-307 (2017-10-12)
Background: During early prenatal stages of brain development, serotonin (5-HT)-specific neurons migrate through somal translocation to form the raphe nuclei and subsequently begin to project to their target regions. The rostral cluster of cells, comprising the median and dorsal raphe
Rebecca L Lowery et al.
International journal of molecular sciences, 22(4) (2021-03-07)
While microglia have been established as critical mediators of synaptic plasticity, the molecular signals underlying this process are still being uncovered. Increasing evidence suggests that microglia utilize these signals in a temporally and regionally heterogeneous manner. Subsequently, it is necessary
R Ye et al.
Genes, brain, and behavior, 13(3), 261-275 (2014-01-11)
Presynaptic serotonin (5-hydroxytryptamine, 5-HT) transporters (SERT) regulate 5-HT signaling via antidepressant-sensitive clearance of released neurotransmitter. Polymorphisms in the human SERT gene (SLC6A4) have been linked to risk for multiple neuropsychiatric disorders, including depression, obsessive-compulsive disorder and autism. Using BXD recombinant

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