推薦產品
生物源
mouse
品質等級
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
4C7, monoclonal
分子量
calculated mol wt 47.23 kDa
observed mol wt ~50 kDa
純化經由
using protein G
物種活性
human, chicken, mouse
包裝
antibody small pack of 100
技術
electron microscopy: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同型
IgG1κ
表位序列
Unknown
Protein ID登錄號
UniProt登錄號
儲存溫度
2-8°C
基因資訊
chicken ... HNF-3beta(395539)
特異性
Clone 4C7 is a mouse monoclonal antibody that detects Hepatocyte nuclear factor-3β (HNF-3β/FoxA2).
免疫原
Recombinant Chicken Rhodopsin (1-6).
應用
Quality Control Testing
Evaluated by Western Blotting in HepG2 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected FoxA2/HNF-3b, in HepG2 cell lysate.
Tested Applications
Electrophoretic Mobility Shift Assay: A representative lot detected FoxA2/HNF-3b in Electrophoretic Mobility Shift Assay (EMSA) (Foucher, I., et al. (2003). Development. 130(9): 1867-76).
Immunofluorescence Analysis: A representative lot detected FoxA2/HNF-3b in Immunofluorescence applications (Camp, D., et al. (2014). Development. 141(20): 3879-88; Hoelzl, M.A., et al. (2017). Dev Biol. 429(1): 132-146).
Immunoprecipitation Analysis: A representative lot immunoprecipitated FoxA2/HNF-3b in Immunoprecipitation applications (van den Brink, G.R., et al. (2001). Gastroenterology. 121(2):317-28).
Immunohistochemistry Applications: A representative lot detected FoxA2/HNF-3b in Immunohistochemistry applications (Ericson, J., et al. (1996). Cell. 87(4):661-73; Kwon, O., et al. (1998). J Clin Invest. 101(10):2054-64; Rousa, F.M., et al. (2000). Mol Cell Biol. 20(21):8264-82; van den Brink, G.R., et al. (2001). Gastroenterology. 121(2):317-28; Camp, D., et al. (2014). Development. 141(20):3879-88; Hoelzl, M.A., et al. (2017). Dev Biol. 429(1):132-146; Amarnath, S., et al. (2017). J Cell Sci. 130(1):119-131).
Immunocytochemistry Analysis: A representative lot detected FoxA2/HNF-3b in Immunohistochemistry applications (Nagase, T., et al. (2009). Dev Dyn. 238(5):1118-30).
Western Blotting Analysis: A representative lot detected FoxA2/HNF-3b in Western Blotting applications (Rousa, F.M., et al. (2000). Mol Cell Biol. 20(21):8264-82; van den Brink, G.R., et al. (2001). Gastroenterology. 121(2):317-28).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting in HepG2 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected FoxA2/HNF-3b, in HepG2 cell lysate.
Tested Applications
Electrophoretic Mobility Shift Assay: A representative lot detected FoxA2/HNF-3b in Electrophoretic Mobility Shift Assay (EMSA) (Foucher, I., et al. (2003). Development. 130(9): 1867-76).
Immunofluorescence Analysis: A representative lot detected FoxA2/HNF-3b in Immunofluorescence applications (Camp, D., et al. (2014). Development. 141(20): 3879-88; Hoelzl, M.A., et al. (2017). Dev Biol. 429(1): 132-146).
Immunoprecipitation Analysis: A representative lot immunoprecipitated FoxA2/HNF-3b in Immunoprecipitation applications (van den Brink, G.R., et al. (2001). Gastroenterology. 121(2):317-28).
Immunohistochemistry Applications: A representative lot detected FoxA2/HNF-3b in Immunohistochemistry applications (Ericson, J., et al. (1996). Cell. 87(4):661-73; Kwon, O., et al. (1998). J Clin Invest. 101(10):2054-64; Rousa, F.M., et al. (2000). Mol Cell Biol. 20(21):8264-82; van den Brink, G.R., et al. (2001). Gastroenterology. 121(2):317-28; Camp, D., et al. (2014). Development. 141(20):3879-88; Hoelzl, M.A., et al. (2017). Dev Biol. 429(1):132-146; Amarnath, S., et al. (2017). J Cell Sci. 130(1):119-131).
Immunocytochemistry Analysis: A representative lot detected FoxA2/HNF-3b in Immunohistochemistry applications (Nagase, T., et al. (2009). Dev Dyn. 238(5):1118-30).
Western Blotting Analysis: A representative lot detected FoxA2/HNF-3b in Western Blotting applications (Rousa, F.M., et al. (2000). Mol Cell Biol. 20(21):8264-82; van den Brink, G.R., et al. (2001). Gastroenterology. 121(2):317-28).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
標靶描述
Hepatocyte nuclear factor-3beta (UniProt: Q9PWP8; also known as HNF-3 beta, FOXA2) is encoded by the HNF-3beta gene (Gene ID: 395539) in Chicken. FoxA2 belongs to the forkhead family of transcription factors that uses a modified helix-turn-helix motif to bind DNA as a monomer. Its DNA binding domain is localized in amino acids 151-245. It is expressed very early during development and is involved in the development of several tissues, including liver, pancreas, lung, prostate, and the nervous system. In mouse embryo, FoxA2 expression is initiated during gastrulation in the node, notochord, and floor plate. Its induction in neural plate cells is an early response to notochord-derived signals and its ectopic expression induces floor plate differentiation. In the ventral mesencephalon and cerebellum, it is co-expressed with Engrailed homeoproteins - En1 and En2, which raises the possibility that the two transcription factors interact to regulate common target genes. FoxA2 expression is observed in different regions of the developing nervous system. Along with FoxA1, it is reported to be crucial for the specification and differentiation of dopamine neurons during embryonic development. FoxA2 also functions as a suppressor of tumor metastasis by inhibition of epithelial-to-mesenchymal transition in human lung cancers. It is shown to be a key target of TGF-β1 in controlling epithelial-to-mesenchymal transition (EMT) in lung cancer cells. (Ref.: Rausa, FM., et al. (2020). Mol. Cell. Biol. 20(21); 8264-8282; Domanskyi, A., et al (2014). Front. Cell. Neurosci. 8; 275; Tang, Y., et al. (2011). Cell Res. 21(2); 316-326; Foucher, I., et al. (2003). Development 130(9); 1867-1876; Ericson, J., et al. (1996). Cell. 87(4); 661-673).
外觀
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
重構
0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
儲存和穩定性
Recommended storage: +2°C to +8°C.
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。
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