ZRB1872

Anti-FOXA2 Antibody, clone 1H12 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 同義詞: Hepatocyte nuclear factor 3-beta;HNF-3-beta;HNF-3B;Forkhead box protein A2;Transcription factor 3B;TCF-3B
• 分類程式碼代碼: 12352203
• NACRES: NA.43

屬性

• 生物源: rabbit
• 品質等級: 200
• 重組細胞: expressed in HEK 293 cells
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 1H12, recombinant monoclonal
• 描述: 1H12 Clone
• 產品線: ZooMAb^® learn more
• 形狀: lyophilized
• 分子量: calculated mol wt 48.31 kDa; observed mol wt ~52 kDa
• 純化經由: using Protein A
• 物種活性: human
• 物種活性（以同源性預測）: rat, canine, feline, monkey, bovine, porcine, mouse
• 包裝: antibody small pack of 25 μL
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• 技術: immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• 同型: IgG
• 表位序列: N-terminal half
• Protein ID登錄號: NP_710141
• UniProt登錄號: Q9Y261
• 環保替代類別: , Aligned
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... FOXA2(3170)

描述

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

特異性

Clone 1H12 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects FOXA2. It targets an epitope within 17 amino acids from the N-terminal half.

免疫原

KLH-conjugated linear peptide corresponding to 17 amino acids from the N-terminal half of human FOXA2.

應用

Quality Control Testing

Evaluated by Western Blotting in A549 cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected FOXA2 in A549 cell lysate.

Tested applications

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected FOXA2 in A549 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected FOXA2 in human colon tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Evaluated by Western Blotting in A549 cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected FOXA2 in A549 cell lysate.

標靶描述

Hepatocyte nuclear factor 3-beta (UniProt: Q9Y261; also known as HNF-3-beta, HNF-3B, Fork head box protein A2, Transcription factor 3B, TCF-3B) is encoded by the FOXA2 (also known as HNF3B, TCF3B) gene (Gene ID: 3170) in human. The FOX family of transcription factors contains more than 50 members, all of which share a winged-helix DNA binding domain. FOXA2 is a fork-head box transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression, and regulation of gene expression in differentiated tissues. It is required for notochord formation and is involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas, and lungs. It contains two transactivation domains (aa 14-93 and 361-457) and its fork-head domain is localized in amino acids 159-252. It shuttles between the nucleus and cytoplasm in a CRM1-dependent manner and in response to insulin signaling via AKT1 it is exported from the nucleus. Phosphorylation of FOXA2 at threonine 156 by AKT inactivates its transcription function and induces its nuclear exclusion. Deletion of FOXA2 in the epithelial cells of the developing mouse lung is shown to inhibit the differentiation of respiratory epithelial cells and causes neonatal respiratory failure, with the characteristics of respiratory distress syndrome in preterm infants. FOXA2 knockdown is reported to induce EMT and increase invasion of A549 lung cancer cells and overexpression of FOXA2 inhibits human lung cancer cell invasion and migration. Two isoforms of FOXA2 have been described that are produced by alternative splicing. This ZooMAbZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Li, J., et al. (2017). Biochemistry. 56(29); 3745-3753; Tang, Y., et al. (2011). Cell Res. 21; 316-326).

外觀

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重構

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律資訊

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable