MABE1128

Anti-SETBP1 Antibody, clone 63

clone 63, from mouse

• 同義詞: SET-binding protein, SEB
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: 63, monoclonal
• 物種活性: human, mouse
• 技術: western blot: suitable
• 同型: IgG2bκ
• NCBI登錄號: NP_001123582
• UniProt登錄號: Q9Y6X0
• 運輸包裝: ambient
• 目標翻譯後修改: unmodified
• 基因資訊: human ... SETBP1(26040)

描述

一般說明

SET-binding protein (UniProt Q9Y6X0; also known as SEB, SETBP1) is encoded by the SETBP1 (also known as KIAA0437, MRD29) gene (Gene ID 26040) in human. SETBP1 is shown to bind the SET nuclear oncogene involved in DNA replication. It is expressed in various tissues with low levels in myeloid and monocytic cells and in CD34+ cells, however, higher expression is observed in myeloid malignancies. Overexpression of SETBP1 promotes leukemogenesis by enhancing full-length SET protein and then impairing the phosphatase activity of the tumor suppressor PP2A through the formation of a SETBP1-SET-PP2A complex. Patients with SETBP1 overexpression have a significantly shorter overall survival, and poor prognosis, particularly in the elderly patients. Mutations in SETBP1 gene are associated with Schinzel-Giedion midface retraction syndrome, which is characterized by severe mental retardation, distinctive facial features, and multiple congenital malformations. (Ref.: Cristobal, I., et al. (2010). Blood 115(3): 615-625).

特異性

Clone 63 targets the N-terminal region of human and mouse SETBP1.

免疫原

GST-tagged recombinant human SETBP1 N-terminal fragment.

應用

Anti-SETBP1, clone 63, Cat. No. MABE1128, is a highly specific mouse monoclonal antibody that targets SETBP1 and has been tested in Western Blotting.

Research Category
Epigenetics & Nuclear Function

Western Blotting Analysis: A 1:4 dilution of clone 63 hybridoma culture supernatant detected SETBP1 in 30-50 µg of REH human acute lymphoid leukemia cell lysate (Courtesy of Jesper Christensen, Ph.D. and Kristian Helin, Ph.D., University of Copenhagen, Denmark).

品質

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected SETBP1 in 10 µg of HeLa cell lysate.

標靶描述

~175 kDa observed. 175.0 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外觀

Protein G purified.

Format: Purified

Purified mouse IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1