MABC1636

Anti-MLKL Antibody, clone 7G2

• 同義詞: Mixed lineage kinase domain-like protein
• 分類程式碼代碼: 12352203
• NACRES: NA.43

屬性

• 生物源: rat
• 品質等級: 200
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 7G2, monoclonal
• 分子量: calculated mol wt 54.58 kDa; observed mol wt ~50 kDa
• 純化經由: using protein G
• 物種活性: human
• 包裝: antibody small pack of 100 μL
• 技術: immunofluorescence: suitable; western blot: suitable
• 同型: IgG2aκ
• 表位序列: C-terminal
• Protein ID登錄號: NP_001135969
• UniProt登錄號: Q8NB16
• 運輸包裝: ambient
• 目標翻譯後修改: unmodified
• 基因資訊: human ... MLKL(197259)

描述

一般說明

Mixed lineage kinase domain-like protein (UniProt: Q8NB16; also known as hMLKL) is encoded by the MLKL gene (Gene ID: 197259) in human. MLKL is a pseudokinase that is a terminal protein in the pro-inflammatory necroptotic cell death program. It plays a critical role in TNFa-induced necroptosis. Although it contains a protein kinase domain (aa 194-469), it lacks several residues that are essential for protein kinase activity. It is activated following phosphorylation at Thr357/Ser358 by RIPK3, which leads to its homotrimerization and localization to the plasma membrane where it binds to highly phosphorylated inositol (InsP6) and cardiolipin and disrupts membrane integrity to cause necrotic cell death. Under basal conditions, MLKL is shown to reside in small puncta that are distributed evenly throughout the cytoplasm, but it is detected in plasma membrane shortly after its phosphorylation. It contains two coiled coil regions (aa 55-84 and 139-180). The second coiled coil region is responsible for its oligomerization. MLKL is also reported to be involved in endosomal trafficking, independently of induction of necroptosis, whereby it enhances degradation of receptors and ligands. Two isoforms of MLKL have been described that are produced by alternative splicing. (Ref.: Samson, AL., et al. (2020). Nat. Commun. 11(1); 3151; Yoon, S., et al. (2017). Immunity. 47(1); 51-65; Wang, H., et al (2014). Mol. Cell 54(1); 133-146).

特異性

Clone 7G2 is a rat monoclonal antibody that detects Human Mixed lineage kinase domain-like protein (MLKL). It targets an epitope within the C-terminal region.

免疫原

GST-tagged recombinant fragment corresponding to 282 amino acids from human Mixed lineage kinase domain-like protein (MLKL) expressed in baculovirus expression system.

應用

Quality Control Testing

Evaluated by Western Blotting in lysates from HT-29 cells.

Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected MLKL in lysates from wild-type HT-29 cells, but not in MLKL knockout cells.

Tested Applications

Western Blotting Analysis: A representative lot detected MLKL in Western Blotting applications (Samson, A.L., et al. (2020). Nat Commun. 11(1):3151).

Immunofluorescence Analysis: A representative lot detected MLKL in Immunofluorescence applications (Samson, A.L., et al. (2020). Nat Commun. 11(1):3151).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-MLKL, clone 7G2, Cat. No. MABC1636, is a rat monoclonal antibody that detects MLKL and is tested for use in Immunofluorescence and Western Blotting.

外觀

Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Recommended storage: +2°C to +8°C.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable