MABC1634

Anti-MLKL Antibody, clone 5A6

• 同義詞: Mixed lineage kinase domain-like protein
• 分類程式碼代碼: 12352203
• NACRES: NA.43

屬性

• 生物源: rat
• 品質等級: 200
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 5A6, monoclonal
• 分子量: calculated mol wt 54.32 kDa; observed mol wt ~54 kDa
• 純化經由: using protein G
• 物種活性: mouse
• 包裝: antibody small pack of 100 μL

描述

一般說明

Mixed lineage kinase domain-like protein (UniProt: Q9D2Y4; also known as MLKL) is encoded by the Mlkl gene (Gene ID: 74568) in murine species. MLKL is a pseudokinase that is a terminal protein in the pro-inflammatory necroptotic cell death program. It plays a critical role in TNFa-induced necroptosis. It is highly expressed in thymus, colon, intestine, liver, spleen, and lung. Its expression is much lower in skeletal muscle, heart and kidney, and it is not detected in the brain. Although it contains a protein kinase domain (aa 192-456), it lacks several residues that are essential for protein kinase activity. It is activated following phosphorylation by RIPK3, which leads to its homotrimerization and localization to the plasma membrane where it binds to highly phosphorylated inositol (InsP6) and disrupts membrane integrity to cause necrotic cell death. Phosphorylation of MLKL at serine 345 is shown to be essential for necroptosis in murine cells. Its interaction with RIPK3 is species specific and mouse MLKL does not interact with human RIPK3. In contrast to human protein, mouse MLKL is not inhibited by necrosulfonamide, because at position 85 it contains a tryptophan residue instead of a cysteine. Under basal conditions, MLKL is shown to reside in small puncta that are distributed evenly throughout the cytoplasm, but it is detected in plasma membrane shortly after its phosphorylation. It contains two coiled coil regions (aa 61-81 and 138-229) and the second coiled coil region is responsible for its oligomerization. Two isoforms of MLKL have been described that are produced by alternative splicing. (Ref.: Samson, AL., et al. (2021). Cell Death Differ. 28(7); 2126-2144; Wang, H., et al (2014). Mol. Cell 54(1); 133-146).

特異性

Clone 5A6 is a rat monoclonal antibody that detects murine Mixed lineage kinase domain-like protein (MLKL). It targets an epitope within the C-terminal region.

免疫原

His-tagged full-length recombinant mouse Mixed lineage kinase domain-like protein (MLKL).

應用

Quality Control Testing

Evaluated by Western Blotting in lysates from Mouse dermal fibroblasts.

Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected MLKL in lysates from wild-type Mouse dermal fibroblasts, but not in lysates from fibroblasts with Mlkl knockout.

Tested Applications

Immunofluorescence Analysis: A representative lot detected MLKL in Immunofluorescence applications (Samson, A.L., et al. (2021). Cell Death Differ. 28(7):2126-2144).

Western Blotting Analysis: A representative lot detected MLKL in Western Blotting applications (Samson, A.L., et al. (2021). Cell Death Differ. 28(7):2126-2144).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-MLKL, clone 5A6, Cat. No. MABC1634, is a rat monoclonal antibody that detects MLKL and is tested for use in Immunofluorescence and Western Blotting.

外觀

Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide

儲存和穩定性

Recommended storage: +2°C to +8°C.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable