推薦產品
生物源
mouse
品質等級
抗體表格
ascites fluid
抗體產品種類
primary antibodies
無性繁殖
COLL-II, monoclonal
物種活性
sheep, human, bovine, rat, pig, mouse, canine
製造商/商標名
Chemicon®
技術
ELISA: suitable
immunohistochemistry: suitable
同型
IgG1
NCBI登錄號
UniProt登錄號
運輸包裝
dry ice
目標翻譯後修改
unmodified
基因資訊
human ... COL2A1(1280)
特異性
Reacts with both pepsin-solubilized and CNBr-cleaved human and bovine collagen type II. No cross-reactivity with collagen types I, III, V and IX.
免疫原
Human (cartilage specific) CNBr-cleaved collagen II.
應用
Immunofluorescence: 1:2-1:5 with fresh, frozen or acetone-fixed material. Mild pepsin digestion is recommended to enhance staining.
Western Blot: 1:1000-1:2000 with chemiluminescent detection
ELISA at 1:100-1:500, pepsin treated samples.
Optimal working dilutions must be determined by the end user.
Western Blot: 1:1000-1:2000 with chemiluminescent detection
ELISA at 1:100-1:500, pepsin treated samples.
Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
ECM Proteins
This Anti-Collagen Type II Antibody, clone COLL-II is validated for use in ELISA, IH for the detection of Collagen Type II.
外觀
Unpurified
Ascites fluid containing no preservatives.
儲存和穩定性
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
分析報告
Control
Cartilage in lung or fetus
Cartilage in lung or fetus
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。
ALK1 opposes ALK5/Smad3 signaling and expression of extracellular matrix components in human chondrocytes.
Journal of Bone and Mineral Research null
Alpha-smooth muscle actin expression and structure integrity in chondrogenesis of human mesenchymal stem cells
Cell and Tissue Research, 324, 457-466 (2006)
Chondrogenesis of human mesenchymal stem cells encapsulated in alginate beads.
Journal of Biomedical Materials Research Part A null
Preservation of the chondrocyte's pericellular matrix improves cell-induced cartilage formation.
Journal of Cellular Biochemistry null
Structural studies on cartilage collagen employing limited cleavage and solubilization with pepsin.
Biochemistry, 11, 4903-4909 (1972)
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