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Merck
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重要文件

AB761

Sigma-Aldrich

Anti-Collagen Type II Antibody

Chemicon®, from rabbit

同義詞:

Anti-Anti-ANFH, Anti-Anti-AOM, Anti-Anti-COL11A3, Anti-Anti-SEDC, Anti-Anti-STL1

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41
無性繁殖:
polyclonal
application:
ELISA
ICC
IHC
WB
物種活性:
human
技術:
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
citations:
8

生物源

rabbit

品質等級

抗體表格

affinity isolated antibody

抗體產品種類

primary antibodies

無性繁殖

polyclonal

純化經由

affinity chromatography

物種活性

human

製造商/商標名

Chemicon®

技術

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

human ... COL2A1(1280)

特異性

The antibody reacts with both native and denatured human collagen type II.



Cross Reactivity Percent

Human collagen type II 90%

Human collagen type I, III, IV, V <1%

Human plasma proteins do not interfere with binding to collagen.

免疫原

Collagen type II extracted and purified from human cartilage.

應用

Anti-Collagen Type II Antibody detects level of Collagen Type II & has been published & validated for use in ELISA, WB, IC, IH.
ELISA 1:1000.

Indirect immunofluorescence on cryostat sections or cultured cells 1:10-1:20

Western Blot 1:100

Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Research Sub Category
ECM Proteins

外觀

Rabbit antiserum passed over agarose sorbents with immobilized human blood plasma proteins and human collagen types I, III, IV and V. Antibody was affinity purified on immobilized human collagen type II.

Product is liquid in 0.15M sodium chloride, 10mM sodium phosphate pH 7.5 with 0.1% mannitol and 0.1% dextran as stabilizers. No preservative.

儲存和穩定性

Maintain at 2-8°C for up to 12 months from date of reciept. Remove any nondispersed aggregates by microcentrifugation prior to use.

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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象形圖

Health hazard

訊號詞

Danger

危險聲明

危險分類

Repr. 1B

儲存類別代碼

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。

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您可以在文件庫中找到最近購買的產品相關文件。

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Vitalization of porous polyethylene (medpor(?)) with chondrocytes promotes early implant vascularization and incorporation into the host tissue.
Susanne Ehrmantraut,Andreas Naumann,Vivienne Willnecker,Stephanie Akinyemi et al.
Tissue Engineering: Part A null
Gabriella Nilsson Hall et al.
Biomaterials, 273, 120820-120820 (2021-04-20)
Tissue engineered constructs have the potential to respond to the unmet medical need of treating deep osteochondral defects. However, current tissue engineering strategies struggle in the attempt to create patterned constructs with biologically distinct functionality. In this work, a developmentally-inspired
Sources of cells that contribute to atherosclerotic intimal calcification: an in vivo genetic fate mapping study.
Naik, V; Leaf, EM; Hu, JH; Yang, HY; Nguyen, NB; Giachelli, CM; Speer, MY
Cardiovascular Research null
Comparative analysis of collagens solubilized from human foetal, and normal and osteoarthritic adult articular cartilage, with emphasis on type VI collagen
Ronziere, M. C. et al.
Biochimica et Biophysica Acta, 1038, 222-230 (1990)
Mu-En Lin et al.
Cardiovascular research, 112(2), 606-616 (2016-09-28)
Vascular smooth muscle cells (SMCs) are major precursors contributing to osteochondrogenesis and calcification in atherosclerosis. Runt-related transcription factor-2 (Runx2) has been found essential for SMC differentiation to an osteochondrogenic phenotype and subsequent calcification in vitro. A recent study using a

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