HTS005M

ChemiSCREEN CCR1 Membrane Preparation

Human CCR1 GPCR membrane preparation for Radioligand binding Assays.

• 分類程式碼代碼: 41106514
• eCl@ss: 32161000
• NACRES: NA.84

屬性

• 生物源: human
• 品質等級: 100
• 重組細胞: expressed in Chem-2 cells
• 製造商／商標名: ChemiScreen; Chemicon^®
• 濃度: 0.5 mg/mL
• 技術: radioligand binding assay (RLBA): suitable
• NCBI登錄號: NM_001295.2
• UniProt登錄號: P32246
• 運輸包裝: dry ice
• 基因資訊: human ... CCR1(1230)

描述

一般說明

CCR1 is a GPCR that binds to a variety of CC ligands, including MIP-1a, RANTES, MCP-3, HCC-1, HCC-2, HCC-4, and MPIF-1 (Olson and Ley, 2002). Lymphocytes, macrophages, dendritic cells, and GM-CSF-activated neutrophils express CCR1 (Kaufmann et al., 2001; Cheng et al., 2001). Two selective, non-peptide small molecule antagonists of CCR1, BX-471 and CP-481,715, have been synthesized (Gladue et al., 2003; Liang et al., 2000). Pharmacological and genetic targeting of CCR1, either alone or in combination with cyclosporin A, reduces cardiac and renal allograft rejection (Gao et al., 2000; Horuk et al., 2001a; Horuk et al., 2001b), allergic encephalomyelitis (Liang et al., 2000), and renal fibrosis (Anders et al., 2002) in experimental models. Chemicon′s CCR1 Membrane Preparations are ideal for screening molecules that disrupt interaction between CCR1 and its ligands. The CCR1 Membrane Preps bind to MIP-1a with a Kd of 1.8 nM, and yield greater than 10-fold signal to background ratio with a MIP-1a concentration of 0.1 nM.

Full-length human CCR1 cDNA

應用

Radioligand binding assay.

生化／生理作用

GPCR Class: A

Protein Target: CCR1

Target Sub-Family: Chemokine

品質

SPECIFICATIONS:
Bmax for [¹²⁵I]MIP-1alpha binding: 3.3 pmol/mg protein
K_d for [¹²⁵I] MIP-1alpha binding: ~ 1.8 nM HOST CELLS:

外觀

Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives.
Packaging method: Membranes protein were adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C.
Each vial typically contains 500 units, where one unit is the amount of membrane prep that yields greater than 1000 cpm specific binding of ¹²⁵I-labeled MIP-1α (0.1 nM input concentration).

儲存和穩定性

Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw.

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2