推薦產品
生物源
mouse
品質等級
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
CHC5.9, monoclonal
物種活性
bovine, amphibian, rat, pig, human
製造商／商標名
Chemicon®
技術
immunohistochemistry: suitable
western blot: suitable
同型
IgM
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... CLTC(1213)
特異性
This antibody reacts with the clathrin heavy chain from a diverse range of species. In nervous tissue it cross-reacts with another coated vesicle polypeptide of 190 kDa which is not present in isolated clathrin triskelions. In some epithelial cells of certain species the antibody may cross-react with a cytokeratin component. It detects coated vesicles from different organs and tissues (e.g. brain, ovary, mammary gland). : Recognizes bovine, rat and amphibian
免疫原
Coated vesicles (clathrin) of bovine brain.
應用
Anti-Clathrin Antibody, clone CHC5.9 is an antibody against Clathrin for use in WB, IH.
Marker for detection of receptor mediated endocytosis
Suitable for frozen sections
Western blotting KNOW SPECIES
Optimal working dilutions must be determined by the end user.
Suitable for frozen sections
Western blotting KNOW SPECIES
Optimal working dilutions must be determined by the end user.
Research Category
Epitope Tags & General Use
Epitope Tags & General Use
Research Sub Category
Organelle & Cell Markers
Organelle & Cell Markers
外觀
Format: Purified
The antibody was purified by gel filtration and is presented as 100μg of lyophilysed material. Reconstitute with 1ml distilled water. Final buffer composition is PBS, pH 7.4.
儲存和穩定性
Maintain at 2-8°C for up to 12 months from date of receipt.
分析報告
Control
Positive reactions using HeLa, SV-40 and RVF-SMC cultured cell lines.
Positive reactions using HeLa, SV-40 and RVF-SMC cultured cell lines.
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
13 - Non Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
Hisako Furukawa et al.
Laboratory investigation; a journal of technical methods and pathology, 84(7), 828-835 (2004-05-04)
Prion protein (PrP) is known to accumulate in some non-neuronal tissues under conditions unrelated to prion diseases. The biochemical and biological nature of such accumulated PrP molecules, however, has not been fully evaluated. In this study, we established experimental myopathy
Molecular links between endocytosis and the actin cytoskeleton.
B Qualmann et al.
The Journal of cell biology, 150(5), F111-F116 (2000-09-07)
J Hirst et al.
Biochimica et biophysica acta, 1404(1-2), 173-193 (1998-08-26)
Clathrin and adaptors are components of clathrin-coated pits and vesicles. The AP-1 adaptor complex is associated with clathrin-coated vesicles budding from the TGN, while the AP-2 adaptor complex is associated with clathrin-coated vesicles budding from the plasma membrane. The clathrin
G Bruder et al.
Experimental cell research, 164(2), 449-462 (1986-06-01)
We have used a monoclonal antibody (CHC5.9) to identify clathrin (Mr 180,000; 'heavy chain') in coated vesicles, triskelion structures prepared in vitro and in high-speed supernatants (HSS) of cell homogenates from a variety of tissues and species (e.g., brain and
Nadezhda V Popova et al.
Journal of neurochemistry, 118(6), 988-998 (2011-07-14)
TPR-containing Rab8b-interacting protein (TRIP8b) is a brain-specific hydrophilic cytosolic protein that contains tetratricopeptide repeats (TPRs). Previous studies revealed interaction of this protein via its TPR-containing domain with Rab8b small GTPase, hyperpolarization-activated cyclic nucleotide-regulated channel (HCN) channels and G protein-coupled receptor
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