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AP124B

Sigma-Aldrich

Goat Anti-Mouse IgG Antibody, biotin-SP conjugate

Chemicon®, from goat

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.46

生物源

goat

品質等級

共軛

biotin conjugate

抗體表格

affinity purified immunoglobulin

抗體產品種類

secondary antibodies

無性繁殖

polyclonal

物種活性

mouse

製造商/商標名

Chemicon®

技術

ELISA: suitable
western blot: suitable

運輸包裝

wet ice

目標翻譯後修改

unmodified

一般說明

Affinity purified goat anti-mouse IgG, Biotin conjugated.
Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

應用

Detect Mouse IgG using this Goat anti-Mouse IgG Antibody, biotin-SP conjugate validated for use in ELISA & WB.
EIA: 1:50,000-1:100,000

Western blots using enzyme-conjugated streptavidin: 1:10,000-1:100,000.

Immunohistochemistry: 1:500-1:5,000

Flow cytometry: 1:200-1:1,000

Fluorescence Immunohisto/cytochemistry: 1:200-1:1,000

Optimal working dilutions must be determined by end user.
Research Category
Secondary & Control Antibodies
Research Sub Category
Whole Immunoglobulin Secondary Antibodies

外觀

ImmunoAffinity Purified
PRESENTATION: Lyophilized. Buffer = 0.02 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA, 0.1% sodium azide.

RECONSTITUTION:

Reconstitute with sterile distilled water to match the volume indicated on the vial label.

儲存和穩定性

Maintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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象形圖

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訊號詞

Warning

危險分類

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3

儲存類別代碼

13 - Non Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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F Mignini et al.
European journal of histochemistry : EJH, 54(2), e17-e17 (2010-06-19)
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Role of ERCC1 promoter hypermethylation in drug resistance to cisplatin in human gliomas.
Hua-Yun Chen,Cui-Jie Shao,Fu-Rong Chen,Aij-Lie Kwan,Zhong-Ping Chen
International Journal of Cancer. Journal International Du Cancer null
Dopamine, vesicular transporters and dopamine receptor expression and localization in rat thymus and spleen.
Fiorenzo Mignini,Daniele Tomassoni,Enea Traini,Francesco Amenta
Journal of Neuroimmunology null
Neurophysiology of supraoptic neurons in C57/BL mice studied in three acute in vitro preparations.
Reza Sharif-Naeini,Sorana Ciura,Tevye J Stachniak,Eric Trudel,Charles W Bourque
Progress in Brain Research null
Lanlan Shen et al.
Journal of the National Cancer Institute, 97(18), 1330-1338 (2005-09-22)
Sporadic colorectal cancers often arise from a region of cells characterized by a "field defect" that has not been well defined molecularly. DNA methylation has been proposed as a candidate mediator of this field defect. The DNA repair gene O6-methylguanine-DNA

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