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重要文件

SML2135

Sigma-Aldrich

生物素基酪酰胺

≥97% (HPLC), powder, tyramide signal amplifier

同義詞:

生物素酚, (3AS,4S,6AR)-六氢-N- [2-(4-羟基苯基)乙基] -2-氧代-1H-噻吩并[3,4-d]咪唑-4-戊

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About This Item

經驗公式(希爾表示法):
C18H25N3O3S
CAS號碼:
分子量::
363.47
MDL號碼:
分類程式碼代碼:
12352200
NACRES:
NA.77

產品名稱

生物素基酪酰胺, ≥97% (HPLC)

化驗

≥97% (HPLC)

形狀

powder

顏色

white to beige

溶解度

DMSO: 2 mg/mL, clear

儲存溫度

−20°C

SMILES 字串

O=C(NCCC1=CC=C(O)C=C1)CCCC[C@@H]2SC[C@@]3([H])[C@]2([H])NC(N3)=O

InChI

1S/C18H25N3O3S/c22-13-7-5-12(6-8-13)9-10-19-16(23)4-2-1-3-15-17-14(11-25-15)20-18(24)21-17/h5-8,14-15,17,22H,1-4,9-11H2,(H,19,23)(H2,20,21,24)/t14-,15-,17-/m0/s1

InChI 密鑰

VZWXNOBHWODXCW-ZOBUZTSGSA-N

應用

生物素酪胺或生物素-苯酚已用于邻近标记法蛋白质鉴定。

生化/生理作用

生物素酪胺是一种酪胺信号放大试剂,适于免疫组织化学(IHC)和原位杂交实验,以及显色和荧光检测。 探针初步结合之后,接着用 HRP 标记的抗体或链霉抗生物素蛋白缀合物进行二次探针检测。 HRP 的催化作用导致在探针附近沉积多个生物素基酪酰胺分子,然后可以用标记的链霉抗生物素蛋白缀合物进行检测。 检测灵敏度可以是传统检测方法的100倍或更高。
用于酪胺信号放大的试剂

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3


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J F Breininger et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 48(12), 1593-1599 (2000-12-02)
To increase the sensitivity of fluorescence in situ hybridization (FISH) for detection of low-abundance mRNAs, we performed FISH on cryostat sections of rat hypothalamus with biotin-labeled riboprobes to leptin receptor (ObRb) and amplified the signal by combining tyramide signal amplification
Nguyen Thi Trang et al.
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, 27(3), 326-331 (2015-04-10)
This study evaluated the sensitivity of biotinyl-tyramide-based in situ hybridization (TISH) method by comparison with chromogenic in situ hybridization (CISH) and immunohistochemical staining (IHC) methods. This study also determined the effect of fixative and fixation time on the detection of
Peiguo Yang et al.
Cell, 181(2), 325-345 (2020-04-18)
The mechanisms underlying ribonucleoprotein (RNP) granule assembly, including the basis for establishing and maintaining RNP granules with distinct composition, are unknown. One prominent type of RNP granule is the stress granule (SG), a dynamic and reversible cytoplasmic assembly formed in
Hagai Marmor-Kollet et al.
Molecular cell, 80(5), 876-891 (2020-11-21)
Stress granules (SGs) are cytoplasmic assemblies of proteins and non-translating mRNAs. Whereas much has been learned about SG formation, a major gap remains in understanding the compositional changes SGs undergo during normal disassembly and under disease conditions. Here, we address
Haiyang Yu et al.
Science (New York, N.Y.), 371(6529) (2020-12-19)
The RNA binding protein TDP-43 forms intranuclear or cytoplasmic aggregates in age-related neurodegenerative diseases. In this study, we found that RNA binding-deficient TDP-43 (produced by neurodegeneration-causing mutations or posttranslational acetylation in its RNA recognition motifs) drove TDP-43 demixing into intranuclear

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