ABS97

Anti-KEAP1 Antibody

from rabbit, purified by affinity chromatography

• 同義詞: Cytosolic inhibitor of Nrf2, Kelch-like protein 19, INrf2
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: mouse, human, rat
• 技術: immunocytochemistry: suitable; western blot: suitable
• NCBI登錄號: NP_036421
• UniProt登錄號: Q14145
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... KEAP1(9817)

描述

一般說明

Anti-oxidant defense is known to be regulated by various mechanisms including the usage of nuclear factor erythroid 2-related factor 2 (Nrf2). Under normal physiological conditions, Nrf2 is bound to Kelch-like ECH-associated protein 1 (Keap1), a cytoplasmic repressor. Keap1 also acts as a substrate adaptor for Cullin3-dependent ubiquitin ligase and also targets Nrf2 for degradation by proteosomes. The substrate adaptor function of Keap1 is inactivated during physiological stress. In this respective condition, a range of oxidative and electrophilic stimuli such as ROS, diethyl malonate, and certain disease processes, resulting from Nrf2 accumulation, enters the nucleus and thereby activates the expression of select anti-oxidant genes. Such genes promote the detoxification of ROS and other harmful molecules, which contribute to the carcinogenesis.

特異性

This antibody recognizes the Kelch 3 region of KEAP1.

免疫原

Epitope: Kelch 3 Region

KLH-conjugated linear peptide corresponding to the Kelch 3 region of human KEAP1.

應用

Anti-KEAP1 Antibody detects level of KEAP1 & has been published & validated for use in WB & IC.

Immunocytochemistry Analysis: 2 µg/mL from a representative lot detected KEAP1 in A431 and HeLa cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
General Post-translation Modification

品質

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected KEAP1 in 10 µg of HeLa cell lysate.

標靶描述

~60-70 kDa observed.
A doublet may be observed in some cell lysates.

外觀

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

分析報告

Control
HeLa cell lysate

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable