ABS2208

Anti-GPR161

• 同義詞: G-protein coupled receptor 161
• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 200
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 分子量: calculated mol wt 60.3 kDa; observed mol wt ~60. kDa
• 純化經由: affinity chromatography
• 物種活性: human
• 物種活性（以同源性預測）: mouse
• 包裝: antibody small pack of 100
• 技術: immunocytochemistry: suitable; immunofluorescence: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• 同型: IgG
• 表位序列: C-terminal
• Protein ID登錄號: NP_001074595
• UniProt登錄號: B2RPY5
• 儲存溫度: -10 to -25°C
• 基因資訊: mouse ... Gpr161(240888)

描述

特異性

This rabbit polyclonal antibody detects G-protein coupled receptor 161 (GPR161). It targets an epitope within 28 amino acids from the C-terminal, cytoplasmic domain.

免疫原

KLH-conjugated linear peptide corresponding to 28 amino acids from the C-terminal, cytoplasmic domain of mouse G-protein coupled receptor 161 (GPR161).

應用

Quality Control Testing

Evaluated by Western Blotting in lysate from HEK293T cell transiently overexpressing human GPR161

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected GPR161 in lysate from HEK293T cell transiently overexpressing human GPR161.

Tested Applications

Immunofluorescence Analysis: A representative lot detected GPR161 in Immunofluorescence application (Shimada, H., et. al. (2017). Cell Rep. 20(2):384-396).

Immunoprecipitation Analysis: A representative lot immunoprecipitated GPR161 in Immunoprecipitation application (Mukhopadhyay, S., et. al. (2013). Cell. 152(1-2):210-23).

Immunocytochemistry Analysis: A representative lot detected GPR161 in Immunocytochemistry application (Mukhopadhyay, S., et. al. (2013). Cell. 152(1-2):210-23).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

標靶描述

G-protein coupled receptor 161 (UniProt: B2RPY5; also known as GPR161) is encoded by the Gpr161 (also known as Gm208) gene (Gene ID: 240888) in murine species. GPR161 is a multi-pass membrane glycoprotein of the GPCR family with seven transmembrane domains. It localizes to the ciliary membrane and is also detected in the recycling endocytic compartment. It is ubiquitously expressed from 8.5 dpc and is mostly concentrated in the developing nervous system at later stages. By 10.5 dpc, it is mainly expressed in the neural tube. At later embryonic stages it is predominantly expressed in the brain, spinal cord, and dorsal ganglia. GPR161 functions as a negative regulator of sonic hedgehog (Shh) signaling, which promotes the processing of Gli3 into its repressive form (Gli3R) during neural tube development via cAMP-protein kinase A signaling. In the presence of Shh, it is removed from primary cilia and is internalized into recycling endosomes, preventing its activity, and allowing activation of the Shh signaling. Gpr161 knockout mice display increased Shh signaling and exhibit embryonic lethality by 10.5 dpc. GPR161 is overexpressed in triple-negative breast cancer and serves is a regulator of mammary epithelial cell proliferation and invasion in a mTOR-dependent manner. Knockdown of GPR161 is reported to impair proliferation of human basal breast cancer cell lines and its overexpression increases cell proliferation, migration, and intracellular accumulation of E-cadherin. Three isoforms of GPR161 have been described that are produced by alternative splicing. (Ref.: Hwang, S-H., et al. (2021). eLife 10:e67121; Mukhopadhyay, S., et al. (2013). Cell 152(1-2); 210-223; Feigin, ME., et al. (2014). Proc. Natl. Acad. Sci. USA. 111(11); 4191-4196).

外觀

Purified rabbit polyclonal antibody in PBS without azide.

重構

0.5 mg/mL Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable