推薦產品
生物源
rabbit
品質等級
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
polyclonal
物種活性
human, mouse
技術
immunohistochemistry: suitable
neutralization: suitable
western blot: suitable
NCBI登錄號
UniProt登錄號
運輸包裝
ambient
目標翻譯後修改
unmodified
基因資訊
human ... SFRP1(6422)
一般說明
Secreted frizzled-related protein 1 (UniProt Q8N474; also known as FRP-1, SARP-2, Secreted apoptosis-related protein 2, sFRP-1) is encoded by the SFRP1 (also known as FRP, FRP1, SARP2) gene (Gene ID 6422) in human. Canonical Wnt signaling is constitutively suppressed in the absence of Wnt, and its activation in the presence of Wnt can be blocked by secreted inhibitors, including the dickkopf-related proteins (DKK) and the secreted frizzled-related proteins (sFRP1-5). sFRPs share homology with the FZD receptor and compete againt FZD for Wnt binding in the extracellular space, sFRPs may also exert their effects by interacting with each other and FZD receptors. sFRP-1 is present during both cardiac development and adult life and is abundantly expressed in the mouse and human heart. Study conducted with Sfrp1-knockout mice indicates a role of sFRP-1 in age-related cardiac deterioration and fibrosis. Human sFRP-1 is produced with a signal peptide sequence (a.a. 1-31), the removal of which yields the mature protein (a.a. 32-314) with an N-terminal FZ (a.a. 53-169) domain, followed by a netrin (NTR) domain (a.a. 186-306).
特異性
This polyclonal antibody neutralized the inhibitory activity of sFRP-1 (Scheel, C., et al. (2011). Cell. 145(6):926 940; Häusler, K.D., et al. (2004). J. Bone Miner. Res. 19(11):1873-1881).
免疫原
Mammalian expressed human sFRP-1, purified from condition culture medium (Häusler, K.D., et al. (2004). J. Bone Miner. Res. 19(11):1873-1881; Uren, A., et al. (2000). J. Biol. Chem. 275(6):4374-4382).
應用
Detect sFRP-1 using this rabbit polyclonal Anti-sFRP-1, Cat. No. ABS1593, validated for use in Immunohistochemistry, Neutralization, and Western Blotting.
Immunohistochemistry Analysis: A 1:260 dilution from a representative lot detected sFRP-1 in human kideny and testis tissue sections (Courtesy of Jeffrey Rubin, M.D., Ph.D., and Aykut Üren, M.D., Ph.D.).
Neutralizing Analysis: A representative lot, in combination with recombinant Wtn5a and an anti-DKK1 antibody, increased migration and mammosphere formation of EpCAM-positive (EPC+) human mammary epithelial cells (MECs) (Scheel, C., et al. (2011). Cell. 145(6):926 940).
Neutralizing Analysis: A representative lot enhanced the formation of TRACP+ mutinucleated and mononucleated cells from co-cultured murine splenic and primary osteoblastic cells upon PGE2 and 1 ,25 (OH)2D3 stimulatation (Häusler, K.D., et al. (2004). J. Bone Miner. Res. 19(11):1873-1881).
Note: Detection of endogenous sFRP-1 in whole cell lysates or tissue samples is difficult without an enrichment step prior to immunoblotting (e.g. by immunoprecipitation or heparin affinity chromatography).
Neutralizing Analysis: A representative lot, in combination with recombinant Wtn5a and an anti-DKK1 antibody, increased migration and mammosphere formation of EpCAM-positive (EPC+) human mammary epithelial cells (MECs) (Scheel, C., et al. (2011). Cell. 145(6):926 940).
Neutralizing Analysis: A representative lot enhanced the formation of TRACP+ mutinucleated and mononucleated cells from co-cultured murine splenic and primary osteoblastic cells upon PGE2 and 1 ,25 (OH)2D3 stimulatation (Häusler, K.D., et al. (2004). J. Bone Miner. Res. 19(11):1873-1881).
Note: Detection of endogenous sFRP-1 in whole cell lysates or tissue samples is difficult without an enrichment step prior to immunoblotting (e.g. by immunoprecipitation or heparin affinity chromatography).
Research Category
Signaling
Signaling
品質
Evaluated by Western Blotting of human sFRP-1 recombinant protein.
Western Blotting Analysis: 1 µg/mL of this antibody detected in 10 ng of mammalian expressed, secreted human sFRP-1.
Western Blotting Analysis: 1 µg/mL of this antibody detected in 10 ng of mammalian expressed, secreted human sFRP-1.
標靶描述
~39 kDa observed. Target band size appears larger than the calculated molecular weights of 32.54/35.39 kDa (mature/pro-form) due to glycosylation. Uncharacterized bands may be observed in some lysate(s).
外觀
Protein A purified.
Format: Purified
Purified rabbit polyclonal antibody in PBS without preservatives.
儲存和穩定性
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
其他說明
Concentration: Please refer to lot specific datasheet.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類(WGK)
WGK 2
閃點(°F)
Not applicable
閃點(°C)
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。
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