跳轉至內容
Merck
全部照片(2)

Key Documents

ABN426

Sigma-Aldrich

Anti-phospho-Neurogranin (Ser36)/Neuromodulin (Ser41) Antibody

serum, from rabbit

同義詞:

Protein kinase C substrate 7.5 kDa protein RC3

登入查看組織和合約定價


About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

rabbit

品質等級

抗體表格

serum

抗體產品種類

primary antibodies

無性繁殖

polyclonal

物種活性

mouse

物種活性(以同源性預測)

rat (based on 100% sequence homology)

技術

western blot: suitable

NCBI登錄號

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

phosphorylation (pSer36)

基因資訊

mouse ... Nrgn(64011)
rat ... Nrgn(64356)

一般說明

Neurogranin (Ng) (also named RC3, p17 or BICKS) is a small protein originally identified in rat brain and abundantly expressed in several telencephalic areas, such as the cerebral cortex, hippocampus, amygdala, and striatum. In neurons, it is found concentrated at dendritic spines where it participates in synaptic signaling events through the regulation of calmodulin (CaM). Neurogranin protein is a critical third messenger and substrate of PKC in the molecular cascade necessary for synaptic development and remodeling. Neurogranin (Ng) features an IQ motif that mediates its interaction with CaM and phosphatidic acid (PA). The interaction is controlled by phosphorylation at serine 36 of Neurogranin. Neurogranin is phosphorylated at serine 36 by PKC. Ser36-phosphorylated Neurogranin is unable to bind either CaM or PA. Neurogranin in localized in the cell bodies of neurons in the cortex and in the apical and basal dendrites of pyramidal neurons. Neurogranin is not found in dendrites and its expression is very low as well in Alzheimer’s disease patients.

特異性

Phosphorylated Neurogranin and Neuromodulin

免疫原

Epitope: IQ domain
KLH-conjugated linear peptide corresponding to the IQ domain of Rat phospho-Neurogranin.

應用

Research Category
Neuroscience
Research Sub Category
Neuroregenerative Medicine
This Anti-phospho-Neurogranin (Ser36)/Neuromodulin (Ser41) Antibody is validated for use in Western Blotting for the detection of phospho-Neurogranin (Ser36)/Neuromodulin (Ser41).

品質

Evaluated by Western Blotting in Mouse brain tissue lysate.

Western Blotting Analysis: A 1:1000 dilution of this antibody detected phospho-Neurogranin (Ser36) / Neuromodulin (Ser41) in 10 µg of Mouse brain tissue lysate treate with magnisium (lane1) or PKC (lane2)

標靶描述

~43 kDa is phospho-Neuromodulin (GAP-43) and ~17 KD is phospho-Neurogranin. Uncharacterized band(s) may appear in some lysates.

外觀

Unpurified
Rabbit Polyclonal serum with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

未找到適合的產品?  

試用我們的產品選擇工具.

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。

已經擁有該產品?

您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

Nikolaus A Watson et al.
Nature communications, 11(1), 1684-1684 (2020-04-05)
There are thousands of known cellular phosphorylation sites, but the paucity of ways to identify kinases for particular phosphorylation events remains a major roadblock for understanding kinase signaling. To address this, we here develop a generally applicable method that exploits

我們的科學家團隊在所有研究領域都有豐富的經驗,包括生命科學、材料科學、化學合成、色譜、分析等.

聯絡技術服務