ABE541

Anti-phospho-STAT2 (Tyr689) Antibody

from rabbit, purified by affinity chromatography

• 同義詞: p113
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: human
• 物種活性（以同源性預測）: mouse (based on 100% sequence homology)
• 技術: immunoprecipitation (IP): suitable; western blot: suitable
• NCBI登錄號: NP_064347
• UniProt登錄號: Q9WVL2
• 運輸包裝: wet ice
• 目標翻譯後修改: phosphorylation (pTyr689)
• 基因資訊: human ... STAT2(6773)

描述

一般說明

The Janus kinase (JAK)/signal transducers and activators of transcription (STAT) signaling pathway plays an important role in cell proliferation, cell differentiation, cell migration, and cell death. It is the principal signaling mechanism for a variety of cytokines and growth factors. Constitutive activation or dysregulation of JAK/STAT signaling can result in inflammatory disease, erythrocytosis, gigantism, and leukemia. STAT2 (113 kDa), originally purified from the nuclei of a-interferon-treated cells, is critical to the transcriptional responses induced by type I interferons, IFN-alpha/beta. Knockout mice with a targeted disruption of STAT2 exhibit greater susceptibility to viral infection and altered responses to Type I interferons. Stat2 is rapidly activated by phosphorylation at Tyr690 in response to stimulation by IFN-alpha/beta via assocations with receptor-bound Jak kinases.

特異性

Other homologies: Rat (89% sequence homology).

This antibody recognizes STAT2 phosphorylated at Tyr689.

免疫原

Epitope: Phosphorylated Tyr689.

KLH-conjugated linear peptide corresponding to mouse STAT2 phosphorylated at Tyr689.

應用

Anti-phospho-STAT2 (Tyr689) Antibody is an antibody against phospho-STAT2 (Tyr689) for use in Immunoprecipitation.

Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated phospho-STAT2 (Tyr689) from IFN alpha treated HeLa cell lysate.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Immunological Signaling

品質

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: A 1:200 dilution of this antibody detected phospho-STAT2 (Tyr689) in 10 µg of IFN alpha/gamma treated HeLa cell lysate.

標靶描述

~113 kDa observed. An uncharacterized band may appear at ~260 kDa in some lysates.

外觀

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

分析報告

Control
Untreated and IFN alpha/gamma treated HeLa cell lysate.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable