ABE1409

Anti-Lysine-Specific Demethylase 4C (JMJD2C)

from rabbit, purified by affinity chromatography

• 同義詞: Gene amplified in squamous cell carcinoma 1 protein, GASC-1 protein, JmjC domain-containing histone demethylation protein 3C, Jumonji domain-containing protein 2C, Jmjd2C
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: human, mouse
• 包裝: antibody small pack of 25 μg
• 技術: immunohistochemistry: suitable (paraffin); western blot: suitable
• NCBI登錄號: NP_055876
• UniProt登錄號: Q9H3R0
• 運輸包裝: ambient
• 目標翻譯後修改: unmodified
• 基因資訊: human ... KDM4C(23081)

描述

一般說明

Lysine-specific demethylase 4C (UniProt: Q9H3R0; also known as EC: 1.14.11.-; Gene amplified in squamous cell carcinoma 1 protein, GASC-1 protein, JmjC domain-containing histone demethylation protein 3C, Jumonji domain-containing protein 2C, Jmjd2c) is encoded by the KDM4C (also known as GASC1, JHDM3C, JMJD2C, KIAA0780) gene (Gene ID: 23081) in human. Jmjd2c is an ubiquitously expressed histone demethylase that specifically demethylates Lys-9 and Lys-36 residues of histone H3. It is shown to demethylate only the trimethylated H3 and does not affect mono- and dimethylated residues. It does neither demethylate histone Lys-4 or Lys-27 of histone H3 nor the Lys-20 of histone H4. Human Jmjd2C contains one jumonji N domain (aa 16-58), a catalytic jumonji C domain (aa 144-310), two PHD zinc finger regions (aa 689-865), and two TUDOR methylated-histone binding domains (aa 877-991). Jmjd2c, along with its closely demethylase Jmjd2b, plays a vital role in self-renewal of embryonic stem cells (ESC) and in generation of induced pluripotent stem cells. Full-length Jmjd2b and Jmjd2c are shown to restore the normal ESC growth phenotype, ESC colony morphology, and also SSEA-1 expression. Jmjd2c is reported to assist polycomb repressive complex (PRC) in transcriptional repression. It is overexpressed in several esophageal squamous cell carcinomas.

特異性

This polyclonal antibody detects Lysine-specific demethylase 4C in multiple human and mouse cell types.

免疫原

KLH-Conjugated linear peptide corresponding to 18 amino acids from the N-terminal half of human Lysine-specific demethylase 4C.

應用

Anti-Lysine-Specific Demethylase 4C (JMJD2C) Antibody, Cat. No. ABE1409, is a highly specific rabbit polyclonal antibody that targets Lysine-specific demethylase 4C and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Research Category
Epigenetics & Nuclear Function

Western Blotting Analysis: 2 µg/mL from a representative lot detected Lysine-Specific Demethylase 4C (JMJD2C) in 10 µg of C2C12 and HEK293 nuclear extract.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Lysine-Specific Demethylase 4C (JMJD2C) in human tonsil, human cervical cancer, and human liver cancer tissues.

品質

Evaluated by Western Blotting in BALB/3T3 clone A31 cell nuclear extract.

Western Blotting Analysis: 2 µg/mL of this antibody detected Lysine-Specific Demethylase 4C (JMJD2C) in 10 µg of BALB/3T3 clone A31 nuclear extract.

標靶描述

~120 kDa observed; 119.98 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外觀

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.