P0082

Monoclonal Anti-POU5F1 (Oct4) antibody produced in mouse

~1.0 mg/mL, clone PF-29, purified immunoglobulin, buffered aqueous solution

• 同義詞: Anti-OCT3, Anti-OCT4, Anti-OTF3, Anti-POU domain, class 5, transcription factor 1
• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: mouse
• 共軛: unconjugated
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: PF-29, monoclonal
• 形狀: buffered aqueous solution
• 分子量: antigen ~43 kDa
• 物種活性: human
• 濃度: ~1.0 mg/mL
• 技術: immunocytochemistry: suitable; indirect ELISA: suitable; western blot: 0.5-1 μg/mL using NT2/D1 total cell extract
• UniProt登錄號: Q01860
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... POU5F1(5460)

描述

一般說明

Monoclonal Anti-POU5F1 (Oct4) (mouse IgG2a isotype) is derived from the hybridoma PF-29 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice, immunized with a peptide.

POU5F1 (POU class 5 homeobox 1) gene codes for a transcription factor that binds to octamer sequence. POU5F1 is mapped to human chromosome 6p21.33.

特異性

Monoclonal Anti-POU5F1 (Oct4) recognizes human POU5F1/Oct4.

免疫原

amino acids 20-37 of human POU5F1 peptide.

應用

Monoclonal Anti-POU5F1 (Oct4) antibody produced in mouse may be used in:

• immunoblotting
• immunocytochemistry
• enzyme-linked immunosorbent assay (ELISA)

生化／生理作用

POU5F1 (POU class 5 homeobox 1) controls homeostasis of embryonic cell development and pluripotency. The POU5F1 gene is highly expressed in cancer cells and offers resistance against chemotherapy.

POU5F1 protein functions as an early developmental control gene. This protein has been recognized as fundamental in the maintenance of pluripotency of embryonic stem cells and primordial germ cells. POU5F1 protein has been proposed as a useful marker for germ cell tumors (GCTs) that can exhibit pluripotential, specifically seminoma/ dysgerminoma/germinoma and embryonal carcinoma (EC). POU5F1 has also been established as a marker for extragonadal germ cell tumors (GCT) and metastatic tumors.

外觀

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

儲存和穩定性

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable
• 個人防護裝備: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)