AB1876-I

Anti-VEGF-A Antibody

from rabbit, purified by affinity chromatography

• 同義詞: VEGF-A, Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: rat, mouse
• 技術: immunohistochemistry: suitable (paraffin); western blot: suitable
• NCBI登錄號: NP_001273985
• UniProt登錄號: Q00731
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: mouse ... Vegfa(22339) ; rat ... Vegfa(83785)

描述

一般說明

The human VEGFA, a 45 kDa homodimeric glycoprotein, is one of the most potent angiogenic factors involved in endothelial cell proliferation, migration, and secretion of MMPs. It also serves as an important survival factor for endothelial cells, inhibiting apoptosis. VEGFA belongs to a family that includes VEGFA, VEGFB, VEGFC, VEGFD, and placental growth factor (PLGF). In humans four prominent isoforms of VEGFA are recognized, VEGF121, VEGF165, VEGF189, and VEGF206. In addition, two other less frequent splice variants, VEGF145 and VEGF183, have also been reported. The properties of native VEGF are very similar to VEGF165. Of all the different isoforms of VEGF, the VEGF165 has the highest bioavailability and biological potency. VEGFA action is mediated by VEGF receptor-1 (VEGFR-1) and VEGFR-2. VEGFR-1 (180 kDa, Flt-1) expression is upregulated by hypoxic conditions in the inner core of tumor mass. VEGFR-2 (200 kDa, KDR) is a major mediator of mitogenic and angiogenic permeability enhancing effects of VEGF. It is also reported to mediate survival signals in endothelial cells.

免疫原

Epitope: Near N-terminus

KLH-conjugated linear peptide corresponding to mouse VEGF-A near the N-terminus.

應用

Anti-VEGF-A Antibody is an antibody against VEGF-A for use in Western Blotting and Immunohistochemistry (Paraffin).

Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected VEGF-A in mouse kidney, mouse cerebellum, mouse spleen, and rat kidney tissue.

Research Category
Signaling

Research Sub Category
Metabolic Hormones & Receptors

品質

Evaluated by Western Blotting in mouse lung tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected VEGF-A in 10 µg of mouse lung tissue lysate.

標靶描述

~27 kDa observed

聯結

Replaces: AB1876

外觀

Affinity

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable