推薦產品
生物源
rabbit
品質等級
抗體表格
affinity isolated antibody
抗體產品種類
primary antibodies
無性繁殖
polyclonal
純化經由
affinity chromatography
物種活性
mouse, guinea pig, rat, human, rabbit, avian
製造商／商標名
Chemicon®
技術
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... GRIN2A(2903)
特異性
Western blot analysis of transfected cells shows the antibody recognizes both NR2A and NR2B subunits equally. There may be a very slight recognition of NR2C and NR2D. Western blot analysis of rat brain shows a single band comigrating with NMDAR2A and NMDAR2B expressed in transfected cells. AB1548 has also been used effectively in Western blot of the membrane fraction of rabbit cortex and avian brain homogenates. No cross-reaction is seen with NMDAR1 or other glutamate receptor subunits.
免疫原
Synthetic peptide (LNSCSNRRVYKKMPSIESDV) corresponding to the C-terminus of rat NMDAR2A receptor subunit conjugated to BSA.
應用
Anti-NMDAR2A&B Antibody, pan detects level of NMDAR2A&B & has been published & validated for use in IH(P), IC, IH, IP & WB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Neurotransmitters & Receptors
Western blot analysis can be done at final concentrations of 0.25-0.5 μg/mL; recognizes a single band in membrane preparations of rat hippocampal neurons ~170,000-180,000 under reduced, SDS PAGE. AB1548 can be used for immunocytochemistry using paraformaldehyde or paraformaldehyde/glutaraldehyde fixed tissue with light and electron microscopy. Cryostat and vibratome sections can be used with or without Triton X-100 treatment. Final concentrations recommended are 0.5-1.0 μg/mL.
The antibody can be used for immunopreciptation of detergent solubilized receptor from brain or transfected cells. For most cases 5 μg of antibody is used with immobilized Protein A.
Optimal working dilutions must be determined by end user.
The antibody can be used for immunopreciptation of detergent solubilized receptor from brain or transfected cells. For most cases 5 μg of antibody is used with immobilized Protein A.
Optimal working dilutions must be determined by end user.
外觀
Affinity purified using the immunogen peptide. Lyophilized from PBS containing 1% BSA.
Note: lot 0509010940 was shipped in liquid format; Do NOT add additional water to this lot of material.
Note: lot 0509010940 was shipped in liquid format; Do NOT add additional water to this lot of material.
儲存和穩定性
Maintain lyophilized material at 2-8°C or -20°C. Reconstitute to 500 μL with sterile distilled water. Store reconstituted material frozen (-20°C or -80°C) in undiluted aliquots for up to six months. Avoid repeated thawing and refreezing. Diluted antibody containing 0.1% sodium azide for Western blot analysis can be stored for several weeks and reused repeatedly.
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
未找到適合的產品？
試用我們的產品選擇工具.
儲存類別代碼
10 - Combustible liquids
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
F Conti et al.
Cerebral cortex (New York, N.Y. : 1991), 9(2), 110-120 (1999-04-29)
N-Methyl-D-aspartate (NMDA) receptors play a critical role in many cortical functions and are implicated in several neuropsychiatric diseases. In this study, the cellular expression of the NMDAR1 (NR1) and NMDAR2A and B (NR2A and B) subunits was investigated in the
Vanessa Lage-Rupprecht et al.
eLife, 9 (2020-12-01)
In the rodent olfactory bulb the smooth dendrites of the principal glutamatergic mitral cells (MCs) form reciprocal dendrodendritic synapses with large spines on GABAergic granule cells (GC), where unitary release of glutamate can trigger postsynaptic local activation of voltage-gated Na+-channels
S M Sisodiya et al.
Neuroreport, 11(11), 2601-2606 (2000-08-16)
NMDA receptors (NR) are important in many neurological processes. Using a large series of human brain tissue, we show that the distribution of NR2A/B immunoreactivity varies according to position along a gyrus. For pyramidal neurons in laminae II and III
Proteolysis of the N-methyl-d-aspartate receptor by calpain in situ.
Guttmann, RP; Sokol, S; Baker, DL; Simpkins, KL; Dong, Y; Lynch, DR
Journal of Pharmacology and Experimental Therapeutics null
NMDA inhibitors cause apoptosis of pyramidal neurons in mature piriform cortex: evidence for a nitric oxide-mediated effect involving inhibitory interneurons.
Lijun Zhou,Annie M Welsh,David Chen,Vassilis E Koliatsos
Neuropharmacology null
我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.
聯絡技術服務