推薦產品
重組細胞
expressed in E. coli
品質等級
化驗
≥90% (homogenous band, SDS-PAGE)
用途
sufficient for 250 reactions
5′ exonuclease activity
<2%, 74 °C (1 h incubation in a reaction with 5′-labeled λ/Sca I digest, per unit enzyme)
secondary activity
nicking (none detected)
特點
Difficult Templates/Specialty Enzymes PCR
High Fidelity PCR
dNTPs included
hotstart: no
製造商/商標名
Novagen®
儲存條件
OK to freeze
濃度
2.5 unit/μL
技術
PCR: suitable
輸入
purified DNA
適合性
suitable for PCR
運輸包裝
wet ice
儲存溫度
−20°C
基因資訊
Thermococcus kodakaraensis ... TK_RS00010(3233723)
一般說明
Our analysis has shown that KOD enzymes are an easy choice for fast, accurate and high-yielding PCR. Our molecular biologists work to develop and formulate polymerases offering the highest specificity, fidelity and yield during PCR amplification. In addition, optimized buffer compositions, convenient master mixes and cycling parameters provide additional ease of use and data reproducibility.KOD DNA Polymerase (formerly KOD HiFi DNA Polymerase) is a recombinant form of Thermococcus kodakaraensis KOD1 DNA polymerase. KOD is a high fidelity thermostable DNA polymerase that amplifies target DNA up to 6 kbp with superior accuracy and yield for PCR applications. The enzyme′s 3′→5′ exonuclease-dependent proofreading activity results in a lower PCR mutation frequency than any other commercially available DNA polymerase. The elongation rate and processivity are 5 times and 10 to 15 times higher, respectively, than for Pfu DNA polymerase, resulting in highly accurate and robust yield, in a short reaction time. The enzyme generates blunt-ended PCR products suitable for cloning with the Novagen Perfectly Blunt® and LIC Vector Kits.
應用
特點和優勢
- Higher fidelity than Pfu DNA polymerase excellent for cloning
- Greater yield extension speed is 2X faster than Taq DNA polymerase and 5X faster than Pfu DNA polymerase
- Higher processivity sequential nucleotide polymerization is 10- to 15-fold greater than Pfu and Tli DNA polymerases
- Amplifies plasmid and lambda DNA templates up to 6 kbp
- Amplifies genomic DNA templates up to 2 kbp
- No truncated amplification products
成分
- 250 U KOD DNA Polymerase (2.5 U/μl)
- 1 ml 10X Buffer #1 for KOD DNA Polymerase (pH 8.0)
- 1 ml 10X Buffer #2 for KOD DNA Polymerase (pH 8.8)
- 1 ml 25 mM MgCl
- 1 ml dNTP Mix (2 mM each)
警告
單位定義
法律資訊
訊號詞
Warning
危險聲明
危險分類
Aquatic Chronic 3 - Eye Irrit. 2
儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 2
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。
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文章
The purpose of Hot Start PCR is to inhibit the PCR reaction in order to reduce nonspecific amplification, prevent the formation of primer dimers, and increase product yields.
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