推薦產品
形狀
liquid
製造商／商標名
Calbiochem®
儲存條件
OK to freeze
protect from light
運輸包裝
wet ice
儲存溫度
−20°C
相關類別
一般說明
一种由四种磷酸酶抑制剂组成的混合物，可同时抑制丝氨酸/苏氨酸和蛋白酪氨酸磷酸酶。可以1 ml或一组5个1 ml小瓶的形式提供。
應用
Phosphatase Inhibitor Cocktail Set V, 50X has been used:
- as a supplement in the lysis buffer to dissociate metabolically activated one (MMe) bone marrow macrophages (BMDMs) for Western blot
- in radio-immunoprecipitation assay (RIPA) modified buffer to incubate cultured human umbilical vein endothelial cells (HUVECs) for western blot
- in lysis buffer to lyse cultured HUVECs for immunoprecipitation
- in wash buffer to wash the protein complexes
- as a component in 4-(2-hydroxyethyl) piperazine-1-ethanesulfonic acid (HEPES) buffer to homogenize J774 cells to assess the effect of lipopolysaccharides (LPS) incubation on cytosolic phospholipase A2 (cPLA2) activity
生化／生理作用
Protein phosphorylation and dephosphorylation play a crucial role in signal transduction, controlling various cellular processes such as cell division, proliferation, and apoptosis. Protein kinases add a phosphate group to specific serine, threonine, or tyrosine residues, which alters the protein′s conformation and its ability to interact with ligands. Protein phosphatases then remove the phosphate group, restoring the protein to its original dephosphorylated state. When extracting phosphorylated proteins from cell and tissue lysates, it is of utmost importance to preserve their phosphorylation state.
特點和優勢
- Convenient: formulations are ready-to-use, making them easy to use.
- Consistent: high-quality formulations ensure reliable and reproducible performance.
- Flexible: the cocktails are designed to target different categories of phosphatases, offering versatility in experimentation.
成分
Each vial contains 1 ml of aqueous solution with the following components:
- 250 mM Sodium Fluoride
- 50 mM ß-Glycerophosphate (Cat. No. 35675)
- 50 mM Sodium Pyrophosphate Decahydrate
- 50 mM Sodium Orthovanadate
警告
毒性：有毒（F）
外觀
在H₂O中。
重構
初次解冻后，分装并冷冻（-20°C）以进行长期储存。临用前按1：50稀释。
法律資訊
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
訊號詞
Warning
危險聲明
危險分類
Eye Irrit. 2
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
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