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product name
磷酸酶抑制剂混合物套装V，50X, Phosphatase Inhibitor Cocktail Set V is a 50X cocktail of four serine/threonine and tyrosine phosphatase inhibitors.
形狀
liquid
製造商／商標名
Calbiochem®
儲存條件
OK to freeze
protect from light
運輸包裝
wet ice
儲存溫度
−20°C
一般說明
一种由四种磷酸酶抑制剂组成的混合物，可同时抑制丝氨酸/苏氨酸和蛋白酪氨酸磷酸酶。可以1 ml或一组5个1 ml小瓶的形式提供。
應用
磷酸酶抑制剂混合物套装V(50X)的应用实例：
- 加入裂解缓冲液以分离代谢活化(metabolically activated one)骨髓巨噬细胞(BMDM)用于蛋白质免疫印迹
- 加入放射性免疫沉淀(RIPA)改良缓冲液以孵育人脐静脉内皮细胞(HUVEC)培养物用于蛋白质免疫印迹
- 加入裂解缓冲液以裂解HUVEC培养物用于免疫沉淀
- 加入洗涤缓冲液以洗涤蛋白复合物
- 加入4-羟乙基哌嗪乙磺酸(HEPES)缓冲液以均质化J774细胞，用于评估脂多糖(LPS)孵育对细胞质磷脂酶A2(cPLA2)活性的影响
生化／生理作用
蛋白磷酸化和去磷酸化在信号转导中起重要作用，调控细胞分裂、增殖和凋亡等各种细胞过程。蛋白质激酶会给特定位点的丝氨酸、苏氨酸或酪氨酸残基加上磷酸基团，改变蛋白的构象及与配体相互作用的能力。接着蛋白质磷酸酶去除磷酸基团，使蛋白质恢复原本的去磷酸化状态。从细胞和组织裂解物中提取磷酸化蛋白时，保持它们的磷酸化状态至关重要。
特點和優勢
- 方便：即用型制剂，使用便捷。
- 稳定：高品质制剂，保证可靠、可重复性能。
- 多用：该混合物靶向不同类别的磷酸酶，在实验中发挥多功能作用。
成分
每小瓶含1 mL包含下列组分的水溶液：
- 250 mM氟化钠
- 50 mM ß-磷酸甘油（货号35675）
- 50 mM十水焦磷酸钠
- 50 mM原钒酸钠
警告
毒性：有毒（F）
外觀
在H₂O中。
重構
初次解冻后，分装并冷冻（-20°C）以进行长期储存。临用前按1：50稀释。
法律資訊
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
訊號詞
Warning
危險聲明
危險分類
Eye Irrit. 2
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
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