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PPC1010

Sigma-Aldrich

Protease and Phosphatase Inhibitor Cocktail

DMSO solution, for the inhibition of serine, cysteine, acid proteases, aminopeptidase, serine/threonine protein phosphatases and L-isozymes of alkaline phosphatase

同義詞:

蛋白酶和磷酸酶抑制剂混合物

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.54

product name

蛋白酶和磷酸酶抑制剂混合物,

形狀

DMSO solution

品質等級

200

包裝

pkg of 1 ml, 5 ml ×

運輸包裝

wet ice

儲存溫度

−20°C

相關類別

一般說明

蛋白酶和磷酸酶抑制剂混合物，设计用于增加细胞提取物中完整和未修饰蛋白质的产量。蛋白酶和磷酸酶抑制剂混合物抑制丝氨酸、半胱氨酸和酸性蛋白酶，以及氨肽酶。 它还抑制碱性磷酸酶的丝氨酸/苏氨酸蛋白磷酸酶和L-同工酶。 该混合物已经在各种动物组织（例如人胎盘和牛肝）的提取物，以及 A431、CHO和U937细胞的提取物上进行了测试。蛋白酶和磷酸酶抑制剂鸡尾酒以溶于DMSO的即用型溶液提供。

應用

蛋白酶和磷酸酶抑制剂混合物可用作放射性免疫沉淀分析(RIPA)缓冲液：
  • 用于细胞蛋白质提取
  • 裂解纯化的CD4+ T细胞
  • 制备胎盘组织和细胞的蛋白提取物

蛋白酶和磷酸酶抑制剂鸡尾酒已用于蛋白质分离缓冲液。

準備報告

生物提取物中混合物的推荐稀释度为从组织或细胞中提取的每 500mg蛋白质的1ml 混合物。在许多情况下，混合物可以以 1％（v/v）的终浓度使用，每 100ml 提取缓冲液使用1ml鸡尾酒溶液。

其他說明

2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)
本产品仅供研&发使用，不得用于药物、家庭或其他用途。有关危险和安全处理方法的信息，请参阅安全数据说明书。

象形圖

Exclamation mark
GHS07

訊號詞

Warning

危險聲明

H315,H319

危險分類

Eye Irrit. 2 - Skin Irrit. 2

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 2

閃點（°F）

188.6 °F

閃點（°C）

87 °C

分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。

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Familial primary aldosteronism (PA) is rare and mostly diagnosed in early-onset hypertension (HT). However, 'sporadic' bilateral adrenal hyperplasia (BAH) is the most frequent cause of PA and remains without genetic etiology in most cases. Our aim was to investigate new
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Mechanisms of ageing and development, 192, 111374-111374 (2020-10-06)
Fibrosis has been considered as a hallmark of dysfunctional adipose tissue (AT), however the role and mechanisms of fibrosis in the age related AT dysfunction are not yet well characterized. The aim of the study was to investigate the mechanisms
Beta-Arrestin1 Prevents Preeclampsia by Downregulation of Mechanosensitive AT1-B2 Receptor Heteromers
Quitterer U, et al.
Cell, S0092-8674(18), 31406-31406 (2018)
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Experimental neurology, 336, 113531-113531 (2020-11-23)
Stroke is the leading cause of long-term, severe disability worldwide. Immediately after the stroke, endogenous inflammatory processes are upregulated, leading to the local neuroinflammation and the potentiation of brain tissue destruction. The innate immune response is triggered as early as
Graphene quantum dots prevent alpha-synucleinopathy in Parkinson?s disease
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