480005

NDSB-201去垢剂

A non-detergent sulfobetaine. Reported to prevent protein aggregation and facilitate the renaturation of chemically and thermally denatured proteins. Zwitterionic over a wide pH range. Easily removed by dialysis.

• 同義詞: NDSB-201去垢剂, 3-（1-吡啶基）-1-丙烷磺酸盐
• 經驗公式（希爾表示法）: C₈H₁₁NO₃S
• CAS號碼: 15471-17-7
• 分子量：: 201.24
• MDL號碼: MFCD00064468
• 分類程式碼代碼: 12161902
• NACRES: NA.21

屬性

• 品質等級: 100
• 化驗: ≥99% (titration)
• 形狀: powder
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze; desiccated (hygroscopic)
• 顏色: white
• 溶解度: water: 50 mg/mL
• 運輸包裝: ambient
• 儲存溫度: 15-25°C
• InChI: 1S/C8H11NO3S/c10-13(11,12)8-4-7-9-5-2-1-3-6-9/h1-3,5-6H,4,7-8H2
• InChI 密鑰: REEBJQTUIJTGAL-UHFFFAOYSA-N

描述

一般說明

一种非洗涤剂磺基甜菜碱。据报告可防止蛋白聚集并促进化学和热变性蛋白的复性。两性离子在很宽的pH值范围内。易通过透析清除。

非洗涤剂磺基甜菜碱类的成员。与CHAPS和其他两性离子不同，NDSB即使在1M浓度下也不会变性。NDSB已用于提高膜蛋白溶解效率，分离核蛋白，在低离子强度缓冲液中色谱纯化嗜盐蛋白以及结晶蛋白。它们在很宽的pH值范围内是两性离子的，并且在近紫外线范围内没有明显的吸收。据报告可防止蛋白聚集并促进化学和热变性蛋白的复性。NDSB可以通过透析轻松去除。

應用

• The non-detergent sulfobetaine-201 acts as a pharmacological chaperone to promote folding and crystallization of the type II TGF-ß receptor extracellular domain.: This study explores the role of NDSB-201 in enhancing the folding and crystallization of a specific protein domain, highlighting its potential as a stabilizing agent in protein biochemistry (Kiessling et al., 2015).


• Stabilizing additives added during cell lysis aid in the solubilization of recombinant proteins.: Research demonstrated that NDSB-201, among other additives, assists in the solubilization of proteins during cell lysis, suggesting its effectiveness in improving recombinant protein yield (Van Voorhis et al., 2012).


• Investigation of protein refolding using a fractional factorial screen: a study of reagent effects and interactions.: Discusses the impact of various reagents, including NDSB-201, on protein refolding, providing insights into how these additives interact and influence protein structure restoration (Fox et al., 2005).


• Preparation of functional recombinant protein from E. coli using a nondetergent sulfobetaine.: Highlights the utility of NDSB-201 in preparing functional recombinant proteins from E. coli, underscoring its practical applications in molecular biology and biochemistry (Chen et al., 2000).

警告

毒性：刺激性（B）

重構

在复溶后存放于室温。储备液可在室温最长稳定3个月。

其他說明

Goldberg, M.E., et al. 1996.Folding & Design 1, 21.
Vuillard, L., et al. 1995.Biochem.J. 305, 337.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable