454578

Anti-Pan-NMDA NR1 Mouse mAb (R1JHL)

lyophilized, clone R1JHL, Calbiochem^®

• 分類程式碼代碼: 12352203
• NACRES: NA.43

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: culture supernatant
• 抗體產品種類: primary antibodies
• 無性繁殖: R1JHL, monoclonal
• 形狀: lyophilized
• 不包含: preservative
• 物種活性: rat
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze
• 同型: IgG
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified

描述

一般說明

Mouse monoclonal antibody lyophilized from cell culture supernatant. Recognizes the ~115 kDa subunit of the NMDAR (N-methyl-D-aspartate receptor) protein.

Recognizes the ~115 kDa NMDA receptor NR1 subunit in rat cortex tissue extracts.

This Anti-Pan-NMDA NR1 Mouse mAb (R1JHL) is validated for use in Immunoblotting for the detection of Pan-NMDA NR1.

免疫原

Rat

a recombinant fusion protein consisting of amino acids 1-564 of rat NMDA NR1

應用

Immunoblotting (1:300, colorimetric)

Immunoprecipitation (3 µg/200 µg lysate)

警告

Toxicity: Standard Handling (A)

外觀

Lyophilized from cell culture supernatant.

重構

Reconstitute in 50 µl PBS (137 mM NaCl, 7.5 mM Na₂HPO₄, 2.7 mM KCl, 1.5 mM KH₂P0₄, pH 7.4)Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.

分析報告

Positive Control
Rat cortex tissue extracts

其他說明

Does not cross-react with other NMDAR subunits. For immunoblotting, dilute in TBST containing 5% nonfat milk and 0.05% sodium azide. Antibody may be used up to 5 times in immunoblotting experiments without significant loss in signal strength. Store diluted antibody at 4°C between uses. Variables associated with assay conditions will dictate the proper working dilution.

Laurie, D.J., and Seeburg, P.H. 1994. J. Neurosci.14, 3180
Ishii, T., et al. 1993. J. Biol. Chem.268, 2836.
Monyer, H., et al. 1992. Science256, 1217.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable